Human Chordin-like 1/CHRDL1 Antibody

Catalog #: AF1808
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1808
AF1808-SP

Product Details

Citations (2)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Chordin-like 1/CHRDL1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Chordin-like 1/CHRDL1 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Chordin-like 1/CHRDL1
Glu28-Cys457
Accession # NP_001137454

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Chordin-Like 1/CHRDL1 (Catalog # 1808-NR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chordin-like 1/CHRDL1

Chordin-like 1 (CHRDL1) also known as Chordin-like, ventroptin, and neuralin, is a secreted glycoprotein that has significant homology to chordin and acts as a bone morphogenetic protein (BMP) antagonist (1‑3). Human Chordin-Like 1 cDNA encodes a 450 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide. Chordin-Like 1 contains three internal cysteine-rich procollagen repeats (CRs) that are conserved in the spacing of their ten cysteines. The CRs of chordin, especially CR1 and CR3, have been shown to be the functional domains for BMP binding (1). The CR1 and CR3 of Chordin-Like 1 are most similar to CR3 of chordin (1). Chordin-Like 1 and chordin exhibit similar BMP inhibitory activity in vivo and directly interact with BMP-4 in vitro (1, 2). However, Chordin-Like 1 differs in its spatial and temporal expression from chordin. Mouse Chordin-Like 1 is detected in mesenchyme-derived cells such as the dermatome and limb bud, as well as chondrocytes of the developing skeleton later in development. In addition, Chordin-Like 1 is expressed embryonically in the gastrointestinal tract, kidney tubules, neural tube, CNS, cerebellum, derivatives of the neural crest cells, developing hair follicles, and the olfactory bulb where it is expressed in a gradient in the retina (1‑3). Chordin-Like 1 is also expressed in adult marrow stromal cells. Human CHRDL1 and the long form of mouse CHRDL1 share 93% aa sequence identity.

Multiple forms of human Chordin-Like 1 containing small deletions or insertions (including an E insertion between P94 and D95, a GKKAK deletion (aa residues 318‑322), and/or another E insertion between K322 and E323) have been described. Similar isoforms have also been reported for mouse Chordin-Like 1. In addition, a short form of mouse Chordin-Like 1 with a shortened variant C-terminus but with all 3 CR domains intact also exists (1). These multiple forms were attributed to alternative splicing and transcriptional termination sites within the coding region of Chordin-Like 1.

References

Nakayama, N. et al. (2001) Dev. Biol. 232:372.
Sakuta, H. et al. (2001) Science 293:111.
Coffinier, C. et al. (2001) Mech. Dev. 100:119.

Entrez Gene IDs

91851 (Human)

Alternate Names

CHL; Chordin-like 1; chordin-like protein 1; CHRDL1; CRDL1; dA141H5.1; Neuralin 1; neuralin-1; neurogenesin-1; NRLN1; NRLN1neuralin 1; Ventroptin alpha; ventroptin; VOPT

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Chordin-like 1/CHRDL1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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CHRDL1 Regulates Stemness in Glioma Stem-like Cells
Authors: I Berglar, S Hehlgans, A Wehle, C Roth, C Herold-Men, F Rödel, D Kögel, B Linder
Cells, 2022-12-03;11(23):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Chordin-like 1, a bone morphogenetic protein-4 antagonist, is upregulated by hypoxia in human retinal pericytes and plays a role in regulating angiogenesis.
Authors: Kane R, Godson C, O'Brien C
Mol. Vis., 2008-06-20;14(0):1138-48.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot