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Human Chordin-like 2/CHRDL2 Antibody

Catalog #: AF2448 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2448
AF2448-SP
Chordin-like 2/CHRDL2 in Human Breast Cancer Tissue.
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Chordin-like 2/CHRDL2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Chordin-like 2/CHRDL2 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Chordin-like 2/CHRDL2
Arg28-Thr451
Accession # NP_056239
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Chordin-Like 2/CHRDL2
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Chordin-like 2/CHRDL2 antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

Chordin-like 2/CHRDL2 in Human Breast Cancer Tissue. Chordin-like 2/CHRDL2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Goat Anti-Human Chordin-like 2/CHRDL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2448) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chordin-like 2/CHRDL2

CHRDL2, also known as breast tumor novel factor 1 (BNF-1), is a secreted BMP antagonist implicated in chondrocyte development.

Entrez Gene IDs
25884 (Human); 69121 (Mouse)
Alternate Names
BNF-1; Chordin like 2; Chordin-like 2; chordin-like protein 2; CHRDL2

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Reviews for Human Chordin-like 2/CHRDL2 Antibody

Average Rating: 4 (Based on 2 Reviews)

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Human Chordin-like 2/CHRDL2 Antibody
By Anonymous on 03/11/2019
Application: Microarrays Sample Tested: EDTA Plasma Species: Human
Other Human Chordin-like 2/CHRDL2 Antibody AF2448
Human Chordin-like 2/CHRDL2 Antibody
By Anonymous on 10/31/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human