Human CHRM5 Antibody

Catalog #: MAB10323 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10323-100
MAB10323-SP

Detection of CHRM5 in HEK293 Human Cell Line Transfected with Human CHRM5 and eGFP by Flow Cytometry.

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All CHRM5 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CHRM5 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CHRM5 in diect ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 590123

Purification

Protein A or G purified from ascites

Immunogen

NS0 mouse myeloma cell line transfected with human CHRM5
Met1-Pro532
Accession # P08912

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CHRM5 in HEK293 Human Cell Line Transfected with Human CHRM5 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human CHRM5 or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human CHRM5 Monoclonal Antibody (Catalog # MAB10323) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CHRM5

Muscarinic receptors are widely distributed throughout the body and control distinct functions according to location and subtype (M1 - M5). They are predominantly expressed in the parasympathetic nervous system where they exert both inhibitory and excitatory effects. Stimulation of CHRM5 is known to increase cyclic AMP levels. The clinical implications of CHRM5 are unknown but it may be linked to schizophrenia.

Long Name

Cholinergic Receptor Muscarinic 5

Entrez Gene IDs

1133 (Human); 213788 (Mouse); 53949 (Rat)

Alternate Names

cholinergic receptor, muscarinic 5; CHRM5; HM5; M5; M5R; mAChR M5; MGC41838; muscarinic acetylcholine receptor M5