Human Chromogranin A Antibody

Catalog #: MAB90982 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB90982-100
MAB90982-SP

Human Chromogranin A ELISA Standard Curve.

1 Image

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Chromogranin A Antibody Summary

Species Reactivity

Human

Specificity

Detects human Chromogranin A in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 948130

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell line HEK293-derived human Chromogranin A
Met1-Gly457
Accession # P10645

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Chromogranin A Monoclonal Antibody (Catalog # MAB9098).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Chromogranin A DuoSet ELISA Kit (Catalog # DY9098-05) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

View Larger

Human Chromogranin A ELISA Standard Curve. Recombinant Human Chromogranin A protein was serially diluted 2-fold and captured by Mouse Anti-Human Chromogranin A Monoclonal Antibody (Catalog # MAB9098) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Chromogranin A Monoclonal Antibody (Catalog # MAB90982) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chromogranin A

Human Chromogranin A (CHGA) an acidic protein and a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells such as islet beta cell secretory granules in pancreas. Chromogranin A expression has been oberserved in neuroendocrine tumors, medullary thyroid carcinoma, as well as carcinoid tumors.

Entrez Gene IDs

1113 (Human); 12652 (Mouse); 24258 (Rat)

Alternate Names

betagranin (N-terminal fragment of chromogranin A); Betagranin; Catestatin; CGA; CHGA; Chromofungin; chromogranin A (parathyroid secretory protein 1); Chromogranin A; chromogranin-A; Pancreastatin; Parastatin; Parathyroid Secretory Protein 1; Pituitary Secretory Protein I; SP-I