Human CILP-1 C-Terminal Fragment Antibody

Catalog #: AF4477
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4477
AF4477-SP

All CILP-1 Antibodies

Product Details

Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CILP-1 C-Terminal Fragment Antibody Summary

Species Reactivity

Human

Specificity

Detects human CILP‑1 C-Terminal Fragment in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CILP-1 N-terminal peptide is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CILP‑1 C-Terminal Fragment
Arg724-Asn1184
Accession # O75339

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human CILP‑1 C-Terminal Fragment

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CILP-1

The CILP-1 (cartilage intermediate-layer protein 1) gene product is a monomeric glycoprotein precursor of two secreted, proteolytically generated products, a 90 kDa N-terminal CILP-1, and a 62 kDa C-terminal NTPPHase-homolog. It is found in both hyaline and fibrocartilage. Although the C-terminal NTPPHase-homolog (aa 725‑1184) does not show enzyme activity, it may participate in matrix structural integrity. Human CILP-1 C-terminus shares 93% and 94% aa identity with porcine and mouse CILP-1 C-terminus, respectively.

Long Name

Cartilage Intermediate Layer Protein 1

Entrez Gene IDs

8483 (Human); 214425 (Mouse); 315761 (Rat)

Alternate Names

cartilage intermediate layer protein 1 C1; cartilage intermediate layer protein 1 C2; cartilage intermediate layer protein 1; cartilage intermediate layer protein, nucleotide pyrophosphohydrolase; Cartilage intermediate-layer protein; CILP; CILP1; CILP-1; HsT18872

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human CILP-1 C-Terminal Fragment Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Lgr5 and Col22a1 Mark Progenitor Cells in the Lineage toward Juvenile Articular Chondrocytes
Authors: C Feng, WCW Chan, Y Lam, X Wang, P Chen, B Niu, VCW Ng, JC Yeo, S Stricker, KSE Cheah, M Koch, S Mundlos, HH Ng, D Chan
Stem Cell Reports, 2019-09-12;13(4):713-729.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC