Human Claudin-8 Antibody

Catalog #: MAB5275 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5275
MAB5275-SP

Detection of Claudin‑8 in HEK293 Human Cell Line Transfected with Human Claudin-8 by Flow Cytometry.

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All Claudin-8 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Claudin-8 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Claudin‑8. Stains human Claudin-8 transfectants but not irrelevant transfectants.

Source

Monoclonal Mouse IgG₁ Clone # 474210

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

HEK293 human embryonic kidney cell line transfected with human Claudin-8
Met1-Val225 (Ser151Pro)
Accession # P56748

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Claudin‑8 in HEK293 Human Cell Line Transfected with Human Claudin-8 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human Claudin-8 was stained with Mouse Anti-Human Claudin-8 Monoclonal Antibody (Catalog # MAB5275, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Intracellular Staining by Flow Cytometry

Detection of Claudin-8 antibody in Caki-2 Human Cell Line antibody by Flow Cytometry.

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Detection of Claudin‑8 in Caki‑2 Human Cell Line by Flow Cytometry. Caki-2 human clear cell carcinoma epithelial cell line was stained with Mouse Anti-Human Claudin-8 Monoclonal Antibody (Catalog # MAB5275, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Claudin-8

Claudin-8 is a 225 amino acid (aa), 25 kDa four-transmembrane protein in the Claudin family of tight junction proteins. It is selectively expressed in tight junctions in the kidney thin descending limb, distal tubule and collecting duct, but is also found in liver and intestinal tight junctions. It is thought to function as an ion barrier protein. The extracellular portions of human Claudin-8 share 86% and 89% aa identity with corresponding portions of mouse and rat Claudin-8, respectively.

Entrez Gene IDs

9073 (Human); 54420 (Mouse); 304124 (Rat)

Alternate Names

claudin 8; Claudin8; Claudin-8; CLDN8; human CLDN8 gene for claudin-810claudin-8