Human CLC Antibody

Catalog #: MAB962
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB962
MAB962-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CLC Antibody Summary

Species Reactivity

Human

Specificity

Detects human CLC in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) CT-1, rhCNTF, rhIL-6, rhIL-11, rhLIF, or rhOSM is observed.

Source

Monoclonal Mouse IgG_2B Clone # 138815

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human CLC
Leu28-Phe225
Accession # Q9UBD9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human CLC (Catalog # 962-CL)

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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CLC in Human Prostate. CLC was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human CLC Monoclonal Antibody (Catalog # MAB962) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLC

Cardiotrophin-like cytokine (CLC) (also known as novel neurotrophin-1 (NNT-1) and B cell stimulating factor (BSF-3)) is a recently discovered 22-25 kDa member of the IL-6 family of cytokines (1, 2, 3). As such, it is expressed as a long type I cytokine with four alpha -helices in it structure (2). Human CLC is synthesized as a 225 amino acid precursor that contains a 27 aa signal sequence and a 198 aa mature region. It contains one potential N-linked glycosylation site that is apparently utilized, and two distinct binding sites for CNTFR alpha and CLF (4, 5). Although CLC has a signal sequence, it is not secreted unless noncovalently dimerized to either CLF or soluble CNTFR alpha (5, 6). Once dimerized, CLC signals through a tripartite receptor complex composed of gp130, LIFR beta and CNTFR alpha (membrane-bound) (5, 7). Within the IL-6 family, human CLC is most homologous to cardiotrophin-1, sharing approximately 29% amino acid sequence identity (8). Human to mouse, mature CLC is 96% aa identical. CLC is a trophic factor for motor neurons, a stimulator of ACTH release from corticotrophs, and an inducer of IgE synthesis and B cell proliferation (9, 10, 11). Cells known to express CLC include embryonic muscle, lung epithelium, and mesenchyme in various regions (12).

References

Murakami, M. et al. (2004) Growth Factors 22:75.
Ernst, M. & B.J. Jenkins (2004) Trends Genet. 20:23.
Senaldi, G. et al. (1999) Proc.Natl. Acad. Sci. USA 96:11458.
Perret, D. et al. (2003) J. Biol. Chem. 279:43961.
Plun-Favreau, H. et al. (2001) EMBO J. 20:1692.
Elson, G.C.A. et al. (2000) Nat. Neurosci. 3:867.
Lelievre, E. et al. (2001) J. Biol. Chem. 276:22476.
Derouet, D. et al. (2004) Proc. Natl. Acad. Sci. USA 101:4827.
Forger, N.G. et al. (2003) J. Neurosci. 23:8854.
Auernhammer, C.J. et al. (2003) Endocrinology 144:1202.
Senaldi, G. et al. (2002) J. Immunol. 168:5690.
de Bovis, B. et al. (2005) Cell Commun. Signal. 3:1.

Long Name

Cardiotrophin-like Cytokine

Entrez Gene IDs

23529 (Human); 56708 (Mouse); 365395 (Rat)

Alternate Names

B-cell stimulating factor 3; B-cell stimulatory factor 3; BSF-3; BSF3BSF-3; cardiotrophin-like cytokine factor 1; CISS2; CLC; CLCF1; CLCNNT-1; CRLF1 associated cytokine-like factor 1; neurotrophin-1/B-cell stimulating factor-3; NNT-1; NNT1B-cell-stimulating factor 3; Novel neurotrophin-1; NR6

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Citation for Human CLC Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Effect of human very low-density lipoproteins on cardiotrophin-like cytokine factor 1 (CLCF1) activity
Authors: S Pasquin, S Chehboun, A Dejda, Y Meliani, V Savin, GJ Warner, R Bosse, A Tormo, G Mayer, M Sharma, P Sapieha, C Martel, JF Gauchat
Sci Rep, 2018-03-05;8(1):3990.
Applications: Immunoprecipitation, Western Blot