Human CLEC-2A Antibody

Catalog #: MAB7219 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7219
MAB7219-SP

Detection of CLEC-2A in U937 Human Cell Line by Flow Cytometry.

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All CLEC-2A Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CLEC-2A Antibody Summary

Species Reactivity

Human

Specificity

Detects human CLEC-2A in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) CLEC-2D, rhCLEC-2, or rhCD69 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 705518

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CLEC-2A
Trp49-Leu174 (predicted)
Accession # Q6UVW9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CLEC-2A in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human CLEC-2A Monoclonal Antibody (Catalog # MAB7219, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC-2A

CLEC-2A (C-type lectin domain family 2 member A), also known as PILAR and KACL, is an approximately 35 kDa type II transmembrane glycoprotein in the C-type lectin superfamily. It contains one C-type lectin domain in its extracellular region. Alternate splicing of human CLEC-2A generates an additional isoform with a substitution of 37 amino acids (aa) at the C-terminus. CLEC-2A is upregulated on antigen-activated CD8⁺ T cells and enhances T cell proliferation through interactions with CD161. It is also expressed on keratinocytes and binds to NKp65/KLRF2, triggering NK cell activation.

Long Name

C-type Lectin Domain Family 2, Member A

Entrez Gene IDs

387836 (Human)

Alternate Names

CLEC2A C-type lectin domain family 2, member A ; CLEC2A; CLEC-2A; KACL; PILAR; UNQ5792