Human CLEC17A APC-conjugated Antibody

Catalog #: FAB101471A Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB101471A-100

Detection of CLEC17A in HEK293 cells by Flow Cytometry.

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Human CLEC17A APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CLEC17A in direct ELISA.

Source

Monoclonal Mouse IgG₁ Clone # 1064719

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

CHO-derived human CLEC17A
Lys194-Cys378
Accession # Q6ZS10

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

HEK293 cells transfected with Human CLEC17A and eGFP vs. Mouse IgG1

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CLEC17A in HEK293 cells by Flow Cytometry. HEK293 cells transfected with Human CLEC17A and eGFP were stained with either (A) Mouse Anti-Human CLEC17A APC‑conjugated Monoclonal Antibody (Catalog # FAB101471A) or (B) Mouse IgG₁ Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CLEC17A

C-type lectin domain family 17, member A (CLEC17A), also known as Prolectin, is type II transmembrane protein that is expressed mainly on dividing B cells found in the germinal centers of secondary lymphoid organs, including lymph nodes, tonsils, stomach, intestine, appendix and spleen (1, 2). CLEC17A binds preferentially to epithelial rather than to mesenchymal cells, and it behaves as a cell adhesion molecule for epithelial cells (2). It has high specificity towards mannose and was found to form disulfide-linked oligomers (1, 3). Human CLEC17A is synthesized as a 378 amino acid (aa) protein that includes a 172 aa cytoplasmic domain, a 21 aa transmembrane segment, and a 185 aa extracellular domain (ECD). Within the ECD, human CLEC17A shares 84% aa sequence identity with canine CLEC17A. C-type lectins are Ca²⁺-depending sugar-binding proteins that are involved in several immune-related and other physiological functions. Presently, 17 groups within the C-type lectin superfamily have been recognized (4), with more C-type lectins being constantly discovered based on the presence of a conserved 115-130 amino acid domain along their sequences - the C-type carbohydrate recognition domain (CRD). However, for most of the recently identified C-type lectins, their interactions with carbohydrates, intracellular functions and molecular mechanisms still remain unclear (3).

References