Human CLEC2L Antibody

Catalog #: MAB10204 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB10204-100
MAB10204-SP

Detection of CLEC2L in HEK293 Human Cell Line Transfected with Human CLEC2L and eGFP by Flow Cytometry

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All CLEC2L Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CLEC2L Antibody Summary

Species Reactivity

Human

Specificity

Detects human CLEC2L in direct ELISA.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2326G

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell, HEK293-derived human CLEC2L protein
Ser90-Thr214
Accession # P0C7M8-1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.5 µg/10⁶ cells

HEK293 human embryonic kidney cell line transfected with human CLEC2L and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CLEC2L in HEK293 Human Cell Line Transfected with Human CLEC2L and eGFP by Flow Cytometry HEK293 human embryonic kidney cell line transfected with human CLEC2L and eGFP was stained with (A) Rabbit Anti-Human CLEC2L Monoclonal Antibody (Catalog # MAB2531) or (B) Rabbit IgG control antibody (MAB1050) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC2L

C-type lectin domain family 2, member L (CLEC2L), is a type II transmembrane protein of the CLEC2 family of proteins that includes CD69, LLT1, AICL and KACL (1, 2). CLEC2 family members have broad tissue expression, with LLT1, AICL and KACL expressed on B cells, monocytes, and keratinocytes, respectively (3-5). In contrast, CLEC2L, a homodimeric cell surface receptor, is predominantly expressed in the brain and is referred to as the brain-associated C-type lectin (BACL) (6). Mature human CLEC2L includes a 68 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 125 aa extracellular domain (ECD). Within the ECD, human CLEC2L shares 94% aa sequence identity with mouse and rat CLEC2L. Our assay shows that CLEC2L binds to Galectin-3.

References