Human CNP Antibody

Catalog #: AF3127 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3127
AF3127-SP

All CNP Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CNP Antibody Summary

Species Reactivity

Human

Specificity

Detects human CNP in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human CNP
Lys24-Cys126
Accession # P23582

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human CNP

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human kidney subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human CNP, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CNP

C-type natriuretic peptide (CNP) belongs to the natriuretic peptide family and functions in an autocrine or paracrine fashion. CNP interacts with the GC-B/NPR-B receptor to promote vasorelaxation, vascular remodeling, and the growth and differentiation of bone and neural tissue. CNP is synthesized as a prohormone that is cleaved intracellularly by furin, yielding a 49 aa propeptide and a 53 aa mature peptide. Additional cleavage of the mature peptide generates 29 aa and 22 aa peptides which have comparable activity in some assays. Within the 103 aa pro CNP, human shares 91% aa sequence identity with mouse and rat pro CNP.

Long Name

C-type Natriuretic Peptide

Entrez Gene IDs

4880 (Human)

Alternate Names

CNP; CNP2; C-type natriuretic peptide; natriuretic peptide C; natriuretic peptide precursor C; NPPC