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Human CNTFR alpha Antibody

Catalog #: AF-303-NA
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF-303-NA
AF-303-SP
CNTF R alpha Enhancement of CNTF-dependent Cell Proliferation and Neutral-ization by Human CNTF R alpha Antibody.
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Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CNTFR alpha Antibody Summary

Species Reactivity
Human
Specificity
Detects human CNTF R alpha  in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CNTF R alpha
Gln23-Pro346 (predicted)
Accession # P26992
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CNTF R alpha (Catalog # 303-CR)
Neutralization
Measured by its ability to neutralize CNTF R alpha -enhanced proliferation in the TF‑1 human erythroleukemic cell line. The Neutralization Dose (ND50) is typically 10-20 µg/mL in the presence of 1 µg/mL Recombinant Human CNTF R alpha and 20 ng/mL Recombinant Human CNTF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization CNTF R alpha Enhancement of CNTF-dependent Cell Proliferation and Neutral-ization by Human CNTF R alpha Antibody. View Larger

CNTF R alpha Enhancement of CNTF-dependent Cell Proliferation and Neutral-ization by Human CNTF R alpha Antibody. Recombinant Human CNTF Ra (Catalog # 303-CR) enhances Recom-binant Human CNTF (Catalog # 257-NT) induced proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human CNTF (20 ng/mL) activity elicited by Recom-binant Human CNTF Ra (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CNTF Ra Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF-303-NA). The ND50 is typically 10-20 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CNTFR alpha

The high-affinity CNTF receptor complex, which mediates the biological action of CNTF, contains three proteins: the ligand-binding alpha  subunit (CNTF R alpha ), and the two signal-transducing proteins LIF R beta and gp130. Whereas LIF R beta and gp130 are widely expressed in many cell types, the expression of CNTF R alpha is restricted to the central and peripheral nervous systems. cDNAs encoding human and rat CNTF R alpha share 94% amino acid (aa) sequence identity. Human CNTF R alpha cDNA encodes a 372 aa  precursor protein with a 22 aa residue signal peptide and four potential glycoslyation sites. CNTF R alpha differs from other cytokine receptors in that it lacks transmembrane and cytoplasmic domains and is anchored to cell membranes by a glycosylphosphatidylinositol (GPI) linkage. Similar to other GPI-linked proteins, soluble CNTF receptor alpha  (CNTF sR alpha ) can be released from the cell surface by phosphatidylinositol-specific phospholipase C. CNTF sR alpha can be released from skeletal muscle in response to peripheral nerve injury and high concentrations of CNTF sR alpha have also been detected in human cerebrospinal fluid. CNTF sR alpha binds CNTF in solution and the complex can act on cells that express only LIF R beta and gp130 but not CNTF R alpha.

Long Name
Ciliary Neurotrophic Factor Receptor alpha
Entrez Gene IDs
1271 (Human); 12804 (Mouse); 313173 (Rat)
Alternate Names
Ciliary neurotrophic factor receptor subunit alpha;Cntfr; CNTF R alpha; CNTFR alpha; CNTFR; CNTFRa

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Citations for Human CNTFR alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Corneal Endothelial Autocrine VIP Enhances Its Integrity in Stored Human Donor Corneoscleral Explant
    Authors: Shay-Whey M. Koh, Dante Gloria, Joseph Molloy
    Investigative Opthalmology & Visual Science
  2. Corneal Endothelial Cell Integrity in Precut Human Donor Corneas Enhanced by Autocrine Vasoactive Intestinal Peptide
    Authors: Shay-Whey M. Koh, Timothy Coll, Dante Gloria, Nicholas Sprehe
    Cornea
  3. Engineered Interleukin-6-derived cytokines recruit artificial receptor complexes and disclose CNTF signaling via the OSMR
    Authors: Rafii, P;Cruz, PR;Ettich, J;Seibel, C;Padrini, G;Wittich, C;Lang, A;Petzsch, P;Köhrer, K;Moll, JM;Floss, DM;Scheller, J;
    The Journal of biological chemistry
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Sortilin facilitates signaling of ciliary neurotrophic factor and related helical type 1 cytokines targeting the gp130/leukemia inhibitory factor receptor beta heterodimer.
    Authors: Larsen JV, Hansen M, Moller B, Madsen P, Scheller J, Nielsen M, Petersen CM
    Mol. Cell. Biol., 2010-06-28;17(0):4175.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Survival and axonal regeneration of off-center retinal ganglion cells of adult cats are promoted with an anti-glaucoma drug, nipradilol, but not BDNF and CNTF.
    Authors: Yata T, Nakamura M, Sagawa H, Tokita Y, Terasaki H, Watanabe M
    Neuroscience, 2007-07-12;148(1):53-64.
    Species: Feline
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  6. The N-terminal cytokine binding domain of LIFR is required for CNTF binding and signaling.
    Authors: He W, Gong K, Smith DK, Ip NY
    FEBS Lett., 2005-08-15;579(20):4317-23.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Ciliary neurotrophic factor released by corneal endothelium surviving oxidative stress ex vivo.
    Authors: Koh SW
    Invest. Ophthalmol. Vis. Sci., 2002-09-01;43(9):2887-96.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  8. Neurotrophic interactions between neurons and astrocytes following AAV1‐Rheb(S16H) transduction in the hippocampus in vivo
    Authors: Min‐Tae Jeon, Gyeong Joon Moon, Sehwan Kim, Minji Choi, Yong‐Seok Oh, Dong Woon Kim et al.
    British Journal of Pharmacology

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