Human Coagulation Factor III/Tissue Factor Quantikine ELISA
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Summary
Precision
Recovery
Linearity
Data Examples
Product Datasheets
Preparation and Storage
Background
Related Research Areas
Human Coagulation Factor III/Tissue Factor Quantikine ELISA Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Citrate Plasma (50 uL), Urine (50 uL)
Sensitivity
2.05 pg/mL
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
Specificity
Natural and recombinant human Coagulation Factor III
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human Coagulation Factor III/Tissue Factor Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Coagulation Factor III in cell culture supernates, cell lysates, plasma, and urine. It contains NS0-expressed recombinant human Coagulation Factor III and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Coagulation Factor III showed linear curves that were p arallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Coagulation Factor III.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 33.4 | 65.4 | 148 | 71 | 144 | 301 |
| Standard Deviation | 0.78 | 1.81 | 5 | 4.5 | 7.8 | 17.1 |
| CV% | 2.3 | 2.8 | 3.4 | 6.3 | 5.4 | 5.7 |
Recovery
The recovery of Coagulation Factor III spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 104 | 95-110 |
| Citrate Plasma (n=4) | 92 | 86-102 |
| EDTA Plasma (n=4) | 91 | 86-97 |
| Heparin Plasma (n=4) | 95 | 88-109 |
| Urine (n=4) | 102 | 96-112 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Coagulation Factor III were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data
Product Datasheets
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Coagulation Factor III/Tissue Factor
Coagulation Factor III, also known as Tissue Factor (TF), Thromboplastin, and CD142, is the primary initiator of the extrinsic coagulation pathway. It is a transmembrane protein that is consitutively expressed in subendothelial cells throughout the vasculature and is inducible on endothelial cells and monocytes. Damage to the vessel wall exposes Coagulation Factor III to circulating Factor VII, triggering the proteolytic cascade that results in fibrin clot formation. Coagulation Factor III plays an important role in thrombotic diseases, disseminated intravascular coagulation, and atherosclerosis. It also plays a role in non-hemostatic inflammation, angiogenesis, and tumor progression.
Entrez Gene IDs:
2152 (Human); 14066 (Mouse)
Alternate Names:
CD142 antigen; CD142; coagulation factor III (thromboplastin, tissue factor); Coagulation Factor III; F3; FLJ17960; TF; TFA; Thromboplastin; Tissue Factor
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
Assay Procedure
Refer to the product Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
100 µL Assay Diluent
100 µL Standard, Control, or Sample
200 µL Conjugate
200 µL Substrate Solution
50 µL Stop Solution
Citations for Human Coagulation Factor III/Tissue Factor Quantikine ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Blood Clot Dynamics and Fibrinolysis Impairment in Cancer: The Role of Plasma Histones and DNA
Authors: Ullah, M;Mirshahi, S;Valinattaj Omran, A;Aldybiat, I;Crepaux, S;Soria, J;Contant, G;Pocard, M;Mirshahi, M;
Cancers
Species: Human
Sample Types: Plasma
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Inhibition of protein disulfide isomerase with PACMA-31 regulates monocyte tissue factor through transcriptional and posttranscriptional mechanisms
Authors: L Beckmann, J Mäder, M Voigtlaend, F Klingler, A Schulenkor, C Lehr, J Regenhardt, C Bokemeyer, W Ruf, C Rolling, F Langer
Thrombosis Research, 2022-09-30;220(0):48-59.
Species: Human
Sample Types: Cell Lysates
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Tissue factor in cancer-associated thromboembolism: possible mechanisms and clinical applications
Authors: S Koizume, Y Miyagi
British Journal of Cancer, 2022-09-12;0(0):.
Species: Human
Sample Types: Plasma
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Tissue factor and its procoagulant activity on cancer-associated thromboembolism in pancreatic cancer: Comment by Mackman et al
Authors: N Mackman, Y Hisada, SJ Archibald
Cancer Science, 2022-02-07;0(0):.
Species: Human
Sample Types: Cell Lysates
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Different Profiles of Cytokines, Chemokines and Coagulation Mediators Associated with Severity in Brazilian Patients Infected with Dengue Virus
Authors: VE Fiestas So, NR da Costa F, CF Dos Santos, G Corrêa, MDC Cipitelli, M Dornelas R, LJ de Souza, PV Damasco, RV da Cunha, FB Dos Santos, LM de Oliveir, EL de Azeredo
Viruses, 2021-09-08;13(9):.
Species: Human
Sample Types: Plasma
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Bacitracin and Rutin Regulate Tissue Factor Production in Inflammatory Monocytes and Acute Myeloid Leukemia Blasts
Authors: L Beckmann, CC Rolling, M Voigtlände, J Mäder, F Klingler, A Schulenkor, C Lehr, C Bokemeyer, W Ruf, F Langer
Cancers, 2021-08-04;13(16):.
Species: Human
Sample Types: Cell Lysates
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Interleukin-1beta Induces Tissue Factor Expression in A549 Cells via EGFR-Dependent and -Independent Mechanisms
Authors: T Mechelke, F Wittig, R Ramer, B Hinz
International Journal of Molecular Sciences, 2021-06-21;22(12):.
Species: Human
Sample Types: Cell Culture Supernates
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Myeloid cell-derived coagulation tissue factor is associated with renal tubular damage in mice fed an adenine diet
Authors: S Yamakage, Y Oe, E Sato, K Okamoto, A Sekimoto, S Kumakura, H Sato, M Yoshida, T Nagasawa, M Miyazaki, S Ito, N Mackman, N Takahashi
Scientific Reports, 2021-06-09;11(1):12159.
Species: Human
Sample Types: Cell Lysates
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Syndecan-1 Promotes Angiogenesis in Triple-Negative Breast Cancer through the Prognostically Relevant Tissue Factor Pathway and Additional Angiogenic Routes
Authors: E Nassar, N Hassan, EA El-Ghonaim, H Hassan, MS Abdullah, TV Rottke, L Kiesel, B Greve, SA Ibrahim, M Götte
Cancers, 2021-05-12;13(10):.
Species: Human
Sample Types: Cell Culture Supernates
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Postprandial factor VII activation does not increase plasma concentrations of prothrombin fragment 1�+�2 in patients with morbid obesity
Authors: LE Landgrebe, CB Juhl, V Andersen, L Moitinho-S, C Bang, EM Bladbjerg
Thromb. Res., 2020-09-01;196(0):260-267.
Species: Human
Sample Types: Serum
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Aryl Hydrocarbon Receptor Activation and Tissue Factor Induction by Fluid Shear Stress and Indoxyl Sulfate in Endothelial Cells
Authors: G Lano, M Laforêt, C Von Kotze, J Perrin, T Addi, P Brunet, S Poitevin, S Burtey, L Dou
Int J Mol Sci, 2020-03-31;21(7):.
Species: Human
Sample Types: Cell Lysates
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Upregulation of Protease-Activated Receptor 2 Promotes Proliferation and Migration of Human Vascular Smooth Muscle Cells (VSMCs)
Authors: M Wei, Y Liu, M Zheng, L Wang, F Ma, Y Qi, G Liu
Med. Sci. Monit., 2019-11-22;25(0):8854-8862.
Species: Human
Sample Types: Cell Culture Supernates
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Trimethylamine N-oxide promotes tissue factor expression and activity in vascular endothelial cells: A new link between trimethylamine N-oxide and atherosclerotic thrombosis
Authors: X Cheng, X Qiu, Y Liu, C Yuan, X Yang
Thromb. Res., 2019-02-23;177(0):110-116.
Species: Human
Sample Types: Cell Lysates
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Alteration in endothelial permeability occurs in response to the activation of PAR2 by factor Xa but not directly by the TF-factor VIIa complex
Authors: NE Benelhaj, A Maraveyas, S Featherby, MEW Collier, MJ Johnson, C Ettelaie
Thromb. Res., 2019-01-16;175(0):13-20.
Species: Human
Sample Types: Cell Culture Supernates
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Characterization of induced pluripotent stem cell-derived megakaryocyte lysates for potential regenerative applications
Authors: A Baigger, D Eicke, Y Yuzefovych, D Pogozhykh, R Blasczyk, C Figueiredo
J. Cell. Mol. Med., 2018-06-12;0(0):.
Species: Human
Sample Types: Cell Lysates
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Can we use biomarkers of coagulation to predict which patients with foot and ankle injury will develop deep vein thrombosis?
Authors: BA Hickey, A Cleves, R Alikhan, N Pugh, L Nokes, A Perera
Foot Ankle Surg, 2017-08-16;0(0):.
Species: Human
Sample Types: Plasma
Applications: ELISA Capture -
Tissue Factor As a Predictor of Recurrent Venous Thromboembolism in Malignancy: Biomarker Analyses of the CATCH Trial
Authors: Alok A Khorana
J. Clin. Oncol, 2016-12-28;0(0):JCO2016674564.
Species: Human
Sample Types: Serum
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Total circulating microparticle levels are increased in patients with deep infiltrating endometriosis
Authors: J Munrós
Hum. Reprod, 2016-12-17;0(0):.
Species: Human
Sample Types: Plasma
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Increased circulating procoagulant and anticoagulant factors as TF and TFPI according to severity or infecting serotypes in human dengue infection
Authors: E Leal de Az, VE Solórzano, DB de Oliveir, CF Marinho, LJ de Souza, RV da Cunha, PV Damasco, CF Kubelka, LM de-Oliveir
Microbes Infect, 2016-08-31;19(1):62-68.
Species: Human
Sample Types: Plasma
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Tissue factor is induced by interleukin-33 in human endothelial cells: a new link between coagulation and inflammation
Sci Rep, 2016-05-04;6(0):25171.
Species: Human
Sample Types: Cell Lysates
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Loss of DGKepsilon induces endothelial cell activation and death independently of complement activation.
Authors: Bruneau S, Neel M, Roumenina L, Frimat M, Laurent L, Fremeaux-Bacchi V, Fakhouri F
Blood, 2014-12-10;125(6):1038-46.
Species: Human
Sample Types: Cell Culture Supernates
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Arachidonate 15-lipoxygenase enzyme products increase platelet aggregation and thrombin generation.
Authors: Vijil C, Hermansson C, Jeppsson A, Bergstrom G, Hulten L
PLoS ONE, 2014-02-12;9(2):e88546.
Species: Human
Sample Types: Cell Lysates
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Increased metabolite levels of glycolysis and pentose phosphate pathway in rabbit atherosclerotic arteries and hypoxic macrophage.
Authors: Yamashita A, Zhao Y, Matsuura Y, Yamasaki K, Moriguchi-Goto S, Sugita C, Iwakiri T, Okuyama N, Koshimoto C, Kawai K, Tamaki N, Zhao S, Kuge Y, Asada Y
PLoS ONE, 2014-01-23;9(1):e86426.
Species: Human
Sample Types: Cell Culture Supernates
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Tissue factor-bearing exosome secretion from human mechanically stimulated bronchial epithelial cells in vitro and in vivo.
Authors: Park J, Sharif A, Tschumperlin D, Lau L, Limbrey R, Howarth P, Drazen J
J Allergy Clin Immunol, 2012-07-22;130(6):1375-83.
Species: Human
Sample Types: Cell Culture Supernates
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Traditional risk factors and D-dimer predict incident cardiovascular disease events in chronic HIV infection.
Authors: Ford ES, Greenwald JH, Richterman AG
AIDS, 2010-06-19;24(10):1509-17.
Species: Human
Sample Types: Plasma
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Toward engineering a human neoendothelium with circulating progenitor cells.
Authors: Allen JB, Khan S, Lapidos KA, Ameer GA
Stem Cells, 2010-02-01;28(2):318-28.
Species: Human
Sample Types: Cell Lysates
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Human Coagulation Factor III/Tissue Factor Quantikine ELISA
By Anonymous
on 10/31/2023
Sample Tested: Lung cancer tissue,Serum and Plasma
The concentration titration uses a log2 algorithm, and we employed an intermittent dilution method. Although the results were not as expected, the detection indicator is still sensitive.
Human Coagulation Factor III/Tissue Factor Quantikine ELISA
By Anonymous
on 05/12/2017
Sample Tested: Cell Culture Media
Selected cell lines were split into various flasks, centrifuged and supernatant used for ELISA
