Human Coagulation Factor V Antibody

Catalog #: MAB98562 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB98562-100
MAB98562-SP

Human Coagulation Factor V ELISA Standard Curve.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Coagulation Factor V Antibody Summary

Species Reactivity

Human

Specificity

Detects human Coagulation Factor V in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 986237

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human Coagulation Factor V synthetic peptide

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Coagulation Factor V Monoclonal Antibody (Catalog # MAB98563).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human Coagulation Factor V ELISA Standard Curve. Recombinant Human Coagulation Factor V protein was serially diluted 2-fold and captured by Mouse Anti-Human Coagulation Factor V Monoclonal Antibody (Catalog # MAB98563) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Coagulation Factor V Monoclonal Antibody (Catalog # MAB98562) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Coagulation Factor V

Factor V (Coagulation Factor Five) is a 330kDa protein of the coagulation system encoded by the F5 gene. Unlike most other coagulation factors, Factor V is not enzymatically active but functions as a cofactor. Factor V is a central regulator of hemostasis. It serves as a critical cofactor for the pro-thrombinase activity of factor Xa that results in the activation of pro-thrombin to thrombin.

Long Name

Coagulation Factor V

Entrez Gene IDs

2153 (Human); 14067 (Mouse); 304929 (Rat)

Alternate Names

Activated protein C cofactor; Cf5; Cf-5; Coagulation Factor 5; coagulation factor V (proaccelerin, labile factor); coagulation factor V jinjiang A2 domain; Coagulation Factor V; F5; factor V Leiden; Factor V; FVL; PCCF; Proaccelerin, Labile Factor; RPRGL1; THPH2