Human Coagulation Factor XIV/Protein C Antibody

Catalog #: MAB3349 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3349
MAB3349-SP

All Coagulation Factor XIV/Protein C Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Coagulation Factor XIV/Protein C Antibody Summary

Species Reactivity

Human

Specificity

Detects human Coagulation Factor XIV/Protein C in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Factor VII, rhFactor X, rhFactor XI, rhThrombin, rhUPA, rhHGFA, rhKallikrein 1, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or 15 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 390226

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor XIV/Protein C
Ala43-Pro461
Accession # P04070

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Coagulation Factor XIV/Protein C (Catalog # 3349-SE)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Coagulation Factor XIV/Protein C (Catalog # 3349-SE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Coagulation Factor XIV/Protein C

Coagulation factor XIV, also known as Protein C, has anti-coagulation activity by degrading factors VIIIa and Va, which are required for thrombin activation. Protein C deficiency results in hereditary thrombophilia, and a severe recessive form may result in massive thrombosis fatal to patient. The deduced amino acid sequence of human protein C predicts a signal peptide (aa 1‑32), a propeptide (aa 33‑42), and a mature chain (aa 43‑461), which can be converted into two disulfide-linked chains (light: aa 43‑199 and heavy: aa 200‑461). The amino acid sequence of human protein C is 99.8%, 80%, 70%, 69% and 54% identical to that of chimpanzee, canine, rat, mouse and chicken.

Long Name

Vitamin K-dependent Protein C

Entrez Gene IDs

5624 (Human); 19123 (Mouse); 25268 (Rat)

Alternate Names

Anticoagulant protein C; APC; Autoprothrombin IIA; Blood coagulation factor XIV; Coagulation Factor XIV; EC 3.4.21; EC 3.4.21.69; PC; PROC; PROC1; protein C (inactivator of coagulation factors Va and VIIIa); Protein C; vitamin K-dependent protein C