Human Common gamma Chain/IL-2 R gamma Fluorescein-conjugated Antibody

Name: Human Common gamma Chain/IL-2 R gamma Fluorescein-conjugated Antibody
Brand: R&D Systems
SKU: FAB2842F

Catalog #: FAB2842F Datasheet / COA / SDS

Discontinued Product

FAB2842F has been discontinued.

View all Common gamma Chain/IL-2 R gamma products.

Detection of Common gamma Chain/IL‑2 R gamma in Human Blood Lymphocytes by Flow Cytometry.

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All Common gamma Chain/IL-2 R gamma Antibodies

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Human Common gamma Chain/IL-2 R gamma Fluorescein-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Common gamma Chain/IL-2 R gamma in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-2 R alpha, rhIL-2 R beta, rhIL-15 R alpha, recombinant mouse IL-2 R gamma, or recombinant canine IL-2 R alpha is observed. In Western blots, approximately 80% cross-reactivity with rhIL-2 R beta, 50% cross-reactivity with rhIL-15 R alpha and recombinant mouse IL-2 R alpha, and no cross‑reactivity with rhIL-2 R alpha is observed.

Source

Monoclonal Mouse IgG_2B Clone # 633162

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf21-derived recombinant human Common gamma Chain/IL-2 R gamma
Leu23-Asn254
Accession # P31785

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Common gamma Chain/IL‑2 R gamma in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human Common ? Chain/IL-2 R? Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB2842F) or (B) Mouse IgG_2BFluorescein Isotype Control (Catalog # IC0041F). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Common gamma Chain/IL-2 R gamma

The gamma chain of the high affinity functional human IL-2 Receptor complex belongs to the hematopoietin receptor family. IL-2 R gamma is a 369 amino acid (aa) protein consisting of a 22 aa signal sequence, a 232 aa extracellular domain, a 29 aa transmembrane domain and an 86 aa cytoplasmic domain. Although IL-2 R gamma by itself does not bind IL‑2 with any appreciable affinity, it is required for IL-2 Receptor signaling. Besides IL-2, the gamma chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. IL-2 R gamma has been designated the Common gamma Chain ( gamma _c). The site of molecular defects in X-linked SCID (Severe Combined Immunodeficiency) has been mapped to the IL-2 R gamma gene.

References

Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
Noguchi, M. et al. (1993) Science 262:1877.

Long Name

IL-2 Receptor gamma

Entrez Gene IDs

3561 (Human); 16186 (Mouse)

Alternate Names

CD132 antigen; CD132; CIDX; combined immunodeficiency, X-linked; common cytokine receptor gamma chain; Common gamma Chain; common gamma-chain; cytokine receptor common subunit gamma; gamma(c); IL-2 R gamma; IL-2 receptor subunit gamma; IL2R gamma; IL-2R subunit gamma; IL2RG; IL-2RG; IMD4; interleukin 2 receptor, gamma; Interleukin-2 receptor subunit gamma; P64; SCIDX; SCIDX1; severe combined immunodeficiency