Summary
Recovery
Linearity
Data Examples
Product Datasheets
Preparation and Storage
Background
Related Research Areas
Human COMP Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
0.036 ng/mL
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human COMP
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human COMP immunoassay is a 4.5 hour solid phase ELISA designed to measure human COMP in cell culture supernates, cell lysates, serum, and plasma. It contains NS0-expressed recombinant human COMP and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human COMP showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring COMP.
Recovery
The recovery of COMP spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 103 | 95-111 |
| Cell Lysates (n=1) | 102 | 98-108 |
| EDTA Plasma (n=4) | 103 | 86-114 |
| Heparin Plasma (n=4) | 99 | 88-112 |
| Serum (n=4) | 102 | 86-114 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human COMP were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data
Product Datasheets
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: COMP/Thrombospondin-5
COMP (Cartilage Oligomeric Matrix Protein), also known as Thrombospondin-5, is a multidomain calcium binding protein that associates with other extracellular matrix molecules. COMP associates into disulfide-linked homopentamers with a central hub and peripheral globular domains connected by flexible strands. An axial pore is formed by the coiled coil assembly and binds vitamin D3 which is involved in bone and cartilage metabolism. An RGD sequence in the third TSP type-3 repeat mediates chondrocyte attachment via Integrins a5b1 or aVb3. COMP is upregulated in rheumatoid arthritis and osteoarthritis, hepatocellular carcinomas, chronic pancreatitis, and pancreatic carcinomas. Several mutations in COMP are associated with skeletal dysplasias.
Long Name:
Cartilage Oligomeric Matrix Protein
Entrez Gene IDs:
1311 (Human); 12845 (Mouse)
Alternate Names:
cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia1, multiple); cartilage oligomeric matrix protein; cartilage oligomeric matrix protein(pseudoachondroplasia, epiphyseal dysplasia1, multiple); COMP; EDM1; EPD1; MED; MEDMGC131819; MGC149768; PSACH; pseudoachondroplasia (epiphyseal dysplasia 1, multiple); THBS5; Thrombospondin5; Thrombospondin-5; TSP5
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
Assay Procedure
Refer to the product Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
100 µL Assay Diluent
50 µL Standard, Control, or Sample
200 µL Conjugate
200 µL Substrate Solution
50 µL Stop Solution
Citations for Human COMP Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
12
Citations: Showing 1 - 10
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Longitudinal measurement of serum cartilage oligomeric matrix protein can detect the progression of cartilage degeneration in anterior cruciate ligament reconstruction patients
Authors: Nishida, Y;Hashimoto, Y;Orita, K;Nishino, K;Kinoshita, T;Iida, K;Nakamura, H;
Asia-Pacific journal of sports medicine, arthroscopy, rehabilitation and technology
Species: Human
Sample Types: Serum
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The Effect of 13 Weeks Long-Distance Bicycle Riding on Inflammatory Response Indicators Related to Joint Cartilage and Muscle Damage
Authors: HJ Kim, HC Lee
International Journal of Environmental Research and Public Health, 2022-12-06;19(23):.
Species: Human
Sample Types: Plasma, Serum
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Associations between serum biomarkers of cartilage metabolism and serum hyaluronic acid, with risk factors, pain categories, and disease severity in knee osteoarthritis: a pilot study
Authors: C Papaneophy, A Alabajos-C, E Viosca-Her, C Calvis, M Costa, AE Christodou, A Kroushovsk, A Lapithis, VM Lapithi, I Papayianni, A Christou, R Messeguer, C Giannaki, K Felekkis
Bmc Musculoskeletal Disorders, 2022-03-02;23(1):195.
Species: Human
Sample Types: Serum
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Comparison of Extracorporeal Shockwave Therapy with Non-Steroid Anti-Inflammatory Drugs and Intra-Articular Hyaluronic Acid Injection for Early Osteoarthritis of the Knees
Authors: SW Jhan, CJ Wang, KT Wu, KK Siu, JY Ko, WC Huang, WY Chou, JH Cheng
Biomedicines, 2022-01-18;10(2):.
Species: Human
Sample Types: Serum
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Cartilage oligomeric matrix protein is an endogenous &beta-arrestin-2-selective allosteric modulator of AT1 receptor counteracting vascular injury
Authors: Y Fu, Y Huang, Z Yang, Y Chen, J Zheng, C Mao, Z Li, Z Liu, B Yu, T Li, M Wang, C Xu, Y Zhou, G Zhao, Y Jia, W Guo, X Jia, T Zhang, L Li, Z Liu, S Guo, M Ma, H Zhang, B Liu, J Du, W Wang, C Tang, P Gao, Q Xu, X Wang, J Liu, J Sun, W Kong
Cell Research, 2021-01-28;0(0):.
Species: Human
Sample Types: Plasma
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Association of Circulating COMP and YKL-40 as Markers of Metabolic Changes of Cartilage with Adipocytokines in Juvenile Idiopathic Arthritis
Authors: K Winsz-Szcz, K Ku?nik-Tro, A Gruenpeter, M Wojdas, K D?bkowska, K Olczyk
Metabolites, 2020-02-10;10(2):.
Species: Human
Sample Types: Serum
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Resolvin D1 prevents epithelial-mesenchymal transition and reduces the stemness features of hepatocellular carcinoma by inhibiting paracrine of cancer-associated fibroblast-derived COMP
Authors: L Sun, Y Wang, L Wang, B Yao, T Chen, Q Li, Z Liu, R Liu, Y Niu, T Song, Q Liu, K Tu
J. Exp. Clin. Cancer Res., 2019-04-18;38(1):170.
Species: Human
Sample Types:
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Increasing Incidence of Colon Cancer in the Young: Assessing the Tumor Biology
Authors: VN Nfonsam, H Jecius, D Chen, PN Omesiete, AN Ewongwo, E Elquza, AJ Scott, J Jandova
J. Am. Coll. Surg., 2019-04-14;0(0):.
Species: Human
Sample Types: Serum
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Wnt and RUNX2 mediate cartilage breakdown by osteoarthritis synovial fibroblast-derived ADAMTS-7 and -12
Authors: S Pérez-Garc, M Carrión, R Villanueva, T Hermida-Gó, M Fernández-, M Mellado, FJ Blanco, Y Juarranz, RP Gomariz
J. Cell. Mol. Med., 2019-03-22;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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The Effects of Well-Rounded Exercise Program on Systemic Biomarkers Related to Cartilage Metabolism
Authors: M Azukizawa, H Ito, Y Hamamoto, T Fujii, Y Morita, A Okahata, T Tomizawa, M Furu, K Nishitani, S Kuriyama, S Nakamura, H Yoshitomi, T Nakatani, T Tsuboyama, M Hamaguchi, S Matsuda, T Yasuda
Cartilage, 2018-04-12;0(0):1947603518767.
Species: Human
Sample Types: Serum
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Effects of dietary supplementation with a standardized aqueous extract of Terminalia chebula fruit (AyuFlex(�)) on joint mobility, comfort, and functional capacity in healthy overweight subjects: a randomized placebo-controlled clinical trial
Authors: HL Lopez, SM Habowski, JE Sandrock, B Raub, A Kedia, EJ Bruno, TN Ziegenfuss
BMC Complement Altern Med, 2017-10-02;17(1):475.
Species: Human
Sample Types: Serum
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Correlation of serum cartilage oligometric matrix protein (COMP) and interleukin-16 (IL-16) levels with disease severity in primary knee osteoarthritis: A pilot study in a Malaysian population
Authors: E Das Gupta, WR Ng, SF Wong, AK Bhurhanude, SS Yeap
PLoS ONE, 2017-09-14;12(9):e0184802.
Species: Human
Sample Types: Serum
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Human COMP Quantikine ELISA Kit
By Anonymous
on 10/04/2020
Sample Tested: Serum
Human COMP Quantikine ELISA Kit
By Jessica Fernando
on 10/23/2017
Sample Tested: Serum
