Human COMP/Thrombospondin-5 Antibody

Catalog #: MAB3134 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3134
MAB3134-SP

Detection of Human COMP/Thrombospondin‑5 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human COMP/Thrombospondin-5 Antibody Summary

Species Reactivity

Human

Specificity

Detects human COMP/Thrombospondin-5 in direct ELISAs and Western blots. Does not cross‑react with recombinant human Thrombospondin-1, -2, or -4.

Source

Monoclonal Mouse IgG_2B Clone # 360215

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human COMP/Thrombospondin-5
Gln21-Ala757 (Ala256Arg)
Accession # P49747.2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.05 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human COMP/Thrombospondin‑5 by Western Blot. Western blot shows lysates of human cartilage tissue. PVDF membrane was probed with 0.05 µg/mL of Mouse Anti-Human COMP/Thrombospondin-5 Monoclonal Antibody (Catalog # MAB3134) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for COMP/Thrombospondin-5 at approximately 90 and 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: COMP/Thrombospondin-5

Cartilage Oligomeric Matrix Protein (COMP), also known as Thrombospondin-5, is a 110 kDa multidomain calcium binding protein that associates with other extracellular matrix molecules. Thrombospondin-1 and -2 constitute subgroup A and form homotrimers, whereas Thrombospondin-3, -4, and COMP constitute subgroup B and form homopentamers (1-4). The human COMP cDNA encodes a 757 amino acid (aa) precursor that includes a 20 aa signal sequence followed by a non-collagenous coiled-coil domain, four EGF-like repeats, seven TSP type-3 repeats, and a globular TSP C-terminal domain (5). Human COMP shares 86-93% aa sequence identity with rat, mouse, equine, bovine, and canine COMP. Within the TSP type-3 repeats and TSP C-terminal domain, human COMP shares 60%, 61%, 74%, and 80% aa sequence identity with human Thrombospondin-1, -2, -3, and -4, respectively. The coiled coil domain mediates the association of COMP into disulfide-linked homopentamers with a central hub and peripheral globular domains connected by flexible strands (6, 7). An axial pore is formed by the coiled coil assembly and binds vitamin D₃ which is involved in bone and cartilage metabolism (8). An RGD sequence in the third TSP type-3 repeat mediates chondrocyte attachment via Integrin alpha 5 beta 1, although when reduced and in the absence of calcium, attachment is mediated via Integrin alpha V beta 3 (9). COMP is upregulated in rheumatoid arthritis and osteoarthritis, hepatocellular carcinomas, chronic pancreatitis, and pancreatic carcinomas (10-12). Elevated circulating COMP levels are used as a biomarker for early onset of some skeletal disorders (10). Several mutations are associated with skeletal dysplasias, and the most common, a point mutation in the third TSP type-3 repeat, results in diminished calcium binding ability (13, 14).

References

Adams, J.C. and J. Lawler (2004) Int J. Biochem. Cell Biol. 36:961.
Posey, K.L. et al. (2004) Int. J. Biochem. Cell Biol. 36:1005.
Adams, J.C. (2004) Int. J. Biochem. Cell Biol. 36:1102.
Mann, H.H. et al. (2004) J. Biol. Chem. 279:25294.
Newton, G. et al. (1994) Genomics, 24:435.
DiCesare, P. et al. (1995) J. Orthopaedic Res. 13:422.
Efimov, V.P. et al. (1994) FEBS Lett. 341:54.
Ozbek, S. et al. (2002) EMBO J. 21:5960.
Chen, F.H. et al. (2005) J. Biol. Chem. 280:32655.
Wislowska, M. and B. Jablonska (2005) Clin. Rheumatol. 24:278.
Xiao, Y. et al. (2004) J. Gastroenterol. Hepatol. 19:296.
Liao, Q. et al. (2003) Scand. J. Gastroenterol. 38:207.
Kennedy, J. et al. (2005) Eur. J. Hum. Genet. 13:547.
Hou, J. et al. (2000) Cell Calcium 27:309.

Long Name

Cartilage Oligomeric Matrix Protein

Entrez Gene IDs

1311 (Human); 12845 (Mouse)

Alternate Names

cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia1, multiple); cartilage oligomeric matrix protein; cartilage oligomeric matrix protein(pseudoachondroplasia, epiphyseal dysplasia1, multiple); COMP; EDM1; EPD1; MED; MEDMGC131819; MGC149768; PSACH; pseudoachondroplasia (epiphyseal dysplasia 1, multiple); THBS5; Thrombospondin5; Thrombospondin-5; TSP5