Human Complement Component C1s Antibody

Catalog #: MAB2060
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2060
MAB2060-SP

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Citations (1)

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Human Complement Component C1s Antibody Summary

Species Reactivity

Human

Specificity

Detects human Complement Component C1s in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human C1r is observed.

Source

Monoclonal Mouse IgG₁ Clone # 306904

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Complement Component C1s
Glu16-Asp688
Accession # P09871

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Complement Component C1s (Catalog # 2060-SE) under non-reducing conditions only

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Complement Component C1s (Catalog # 2060-SE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Component C1s

The classical complement pathway plays a major role in innate immunity against infection. This pathway is triggered by C1, a multimolecular complex composed of the recognition protein C1q and two serine proteases, C1r and C1s. Following the C1q recognition, C1r is autoactivated, and in turn activates C1s, which cleaves C4 and C2, the C1 substrates (1). Both C1r and C1s activation involve cleavage of a specific Arg-Ile bond, converting single-chain proenzymes into active proteases of disulfide bond-linked chains (A and B) (2). The A chains contain multiple domains in the order of CUB1-EGF-CUB2-CCP1-CCP2-Activation Peptide. The B chains contain the serine protease catalytic domain. The full-length (amino acid residues 1‑688) of human C1s was expressed (3‑5). The purified protein corresponded to the processed active form, with A and B chains starting at residue Glu16 and Ile438, respectively.

References

Arlaud, G.J. et al. (2002) Biochem. Soc. Trans. 30:1001.
Lacroix, M. et al. (2001) J. Biol. Chem. 276:36233.
Tosi, M. et al. (1987) Biochemistry 26:8516.
Mackinnon, C.M. et al. (1987) Eur. J. Biochem. 169:547.
Kusumoto, H. et al. (1988) Proc. Natl. Acad. Sci. USA 85:7307.

Entrez Gene IDs

716 (Human)

Alternate Names

basic proline-rich peptide IB-1; C1 esterase; C1S; complement C1s subcomponent; Complement component 1 subcomponent s; complement component 1, s subcomponent; Complement Component C1s; EC 3.4.21; EC 3.4.21.42; FLJ44757

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Human Complement Component C1s Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1