Human Complement Component C2 Antibody

Catalog #: AF1936
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1936
AF1936-SP

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Citations (1)

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Human Complement Component C2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Complement Component C2 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross‑reactivity with recombinant mouse Complement Component C2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Complement Component C2
Ala21-Leu752
Accession # P06681

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Complement Component C2 (Catalog # 1936-SE)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Complement Component C2 (Catalog # 1936-SE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Component C2

The classical complement pathway plays a major role in innate immunity against infection. This pathway is triggered by C1, a multimolecular complex composed of the recognition protein C1q and two serine proteases, C1r and C1s (1). After activation by C1, the single-chain form of C2 (amino acid residues 21‑752) becomes two chains, which are referred to as C2A and C2B. C2A (residues 244‑752) consists of a vWF domain (residues 254‑452) and a serine protease domain (residues 466‑752). C2B (residues 21‑243) contains 3 Sushi (SCR) domains. C2A, then combines with complement factor 4B to generate the C3 or C5 convertase. The full‑length of human C2 was expressed, and the purified protein corresponded to the single-chain form with the peptidase activity.

References

Arlaud, G.J. et al. (2002) Biochem. Soc. Trans. 30:1001.

Entrez Gene IDs

717 (Human); 12263 (Mouse); 24231 (Rat)

Alternate Names

C2; C3/C5 convertase; CO2; complement C2; complement component 2; Complement Component C2; DKFZp779M0311; EC 3.4.21; EC 3.4.21.43

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Complement Component C2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1