Human Complement Component C9 Antibody

Name: Human Complement Component C9 Antibody
Brand: R&D Systems
SKU: AF8126
Rating: 5 (1 reviews)

Catalog #: AF8126 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF8126
AF8126-SP

Detection of Human Complement Component C9 by Western Blot.

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All Complement Component C9 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Complement Component C9 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Complement Component C9 in directs ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Human embryonic kidney cell line HEK293-derived human Complement Component C9
Gln22-Lys559 (predicted)
Accession # P02748

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Complement Component C9 by Western Blot. Western blot shows lysates of human plasma. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human Complement Component C9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8126) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Complement Component C9 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Component C9

Complement Component 9 (C9) is a 65-70 kDa protein of the complement membrane attack complex (C5b-9) that binds C5b-8, polymerizes, and forms pores upon activation of the complement cascade. Mature human C9 shares 60% and 66% amino acid sequence identity with mouse and rat C9, respectively. It may be proteolytically cleaved by thrombin into a 28-34 kDa cysteine-rich, mannosylated soluble C9a subunit, and a 37-38 kDa membrane-anchored N-glycosylated C9b. These fragments are also able to polymerize.

Entrez Gene IDs

735 (Human); 12279 (Mouse); 117512 (Rat)

Alternate Names

complement component 9; Complement Component C9