Human Complement Factor H Antibody

Catalog #: MAB4779
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4779
MAB4779-SP

Detection of Human Complement Factor H by Western Blot.

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Complement Factor H Antibody Summary

Species Reactivity

Human

Specificity

Detects human Complement Factor H in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 556317

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Complement Factor H
Ser860-Arg1231
Accession # P08603

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Complement Factor H, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Complement Factor H by Western Blot. Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 2 µg/mL of Human Complement Factor H Monoclonal Antibody (Catalog # MAB4779) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Complement Factor H at approximately 155 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Factor H

Complement Factor H (CFH) is a 155 kDa glycoprotein that negatively regulates the alternative pathway complement cascade. It is secreted by Kupffer cells, hepatocytes, vascular endothelial cells, and platelets and circulates in the serum at high concentration. CFH interacts with cell surface polyanions and prevents local complement activation by sequestering complement component C3b, accelerating the decay of C3 and C5, and functioning as a cofactor for Factor I. CFH is composed of 20 SCR (short consensus repeats). Alternate splicing generates an isoform that is truncated following SCR7. Within SCR5-20 (aa 260-1231), human CFH shares 60% and 62% aa sequence identity with mouse and rat CFH, respectively.

Entrez Gene IDs

3075 (Human); 12628 (Mouse); 155012 (Rat)

Alternate Names

adrenomedullin binding protein; age-related maculopathy susceptibility 1; AHUS1; AMBP1; ARMD4; ARMS1; beta-1H; beta-1-H-globulin ; beta-1-H-globulin; CFH; CFHL3; Complement Factor H; factor H; factor H-like 1; FH; FHL1; H factor 1 (complement); H factor 1; H factor 2 (complement); HF; HF1; HF1ARMS1; HF2; HUS; HUSMGC88246

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Complement Factor H Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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CFH exerts anti-oxidant effects on retinal pigment epithelial cells independently from protecting against membrane attack complex
Authors: C Borras, J Canonica, S Jorieux, T Abache, M El Sanhara, C Klein, K Delaunay, L Jonet, M Salvodelli, MC Naud, Y Arsenijevi, A Shalabi, L Souchaud, F Behar-Cohe, V Dinet
Sci Rep, 2019-09-25;9(1):13873.
Species: Human
Sample Types: Whole Cells
Applications: IHC
Developing Potential Candidates of Preclinical Preeclampsia
Authors: Sandra Founds, Xuemei Zeng, David Lykins, James M. Roberts
International Journal of Molecular Sciences