Human Complement Factor MASP3 Catalytic Domain Biotinylated Antibody

Catalog #: BAF1724 Datasheet / COA / SDS

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BAF1724

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Human Complement Factor MASP3 Catalytic Domain Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Complement Factor MASP3 Catalytic Domain in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Complement Factor MASP3 Catalytic Domain
Ile450-Val721
Accession # NP_624302

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Complement MASP3 Catalytic Domain (Catalog # 1724-SE)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Factor MASP3

MASP3 is a member of the MASPs involved in mannan-binding lectin (MBL) complement pathway (1). The MBL pathway is initiated by the binding of MBL to specific carbohydrate structures found on the surface of a variety of microorganisms. Activation of the complement pathway via MBL is initiated by specific MASPs. Three MASPs have been identified and all have domain structures similar to those of C1r and C1s with a heavy chain (chain A) and a light chain (chain B). Chain A is composed of CUB1, EGF, CUB2, CCP1 and CCP2 while chain B corresponds to the catalytic domain found in many serine proteases. MASP1 and MASP3 are two alternatively spliced products of a single gene, which contain the same A chains but entirely different B chains. Distinct MASPs found in different MBL oligomers may have different biological activities. For example, MASP3, found together with MASP2, downregulates the C4 and C2 cleaving activity of MASP2. The protease activity of MASP3 is first revealed here using rhMASP3CD (2), which is inhibited by serine protease inhibitors such as Ecotin and AEBSF (R&D Systems, Catalog # 1328-PI and EI001).

References

Dahl, M.R. et al. (2001) Immunity 15:127.
Cortesio, C.L. and W. Jiang (2006) Arch. Biochem. Biophys. 449:164.

Long Name

Mannan-binding lectin-Associated Serine Protease 3

Entrez Gene IDs

5648 (Human)

Alternate Names

Complement Factor MASP3; Complement factor MASP-3; Complement-activating component of Ra-reactive factor; CRARF1; CRARFMAP1; DKFZp686I01199; EC 3.4.21; EC 3.4.21.-; EC 3.4.21.104; EC 3.4.21.42; FLJ26383; mannan-binding lectin serine peptidase 1 (C4/C2 activating component ofRa-reactive factor); mannan-binding lectin serine protease 1 (C4/C2 activating component ofRa-reactive factor); mannan-binding lectin serine protease 1; Mannose-binding lectin-associated serine protease 1; Mannose-binding protein-associated serine protease; MAp44; MASP; MASP1; MASP-1; MASP3; MGC126283; MGC126284; PRSS5; Ra-reactive factor serine protease p100; RaRF; Serine protease 5