Human COMT Antibody

Catalog #: AF7386 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7386
AF7386-SP

Detection of Human COMT by Western Blot.

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Human COMT Antibody Summary

Species Reactivity

Human

Specificity

Detects human COMT in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human COMT
Gly52-Pro271 (Val158Met)
Accession # P21964

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human COMT by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and human placenta tissue. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human COMT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7386) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for COMT at approximately 25 and 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: COMT

COMT (Catechol-O-MethylTransferase) is a 29-30 kDa member of the COMT family of enzymes. It shares the same acronym with plant COMT, or caffeic acid 3-O-methyltransferase that is involved in phenylpropanoid synthesis. Mammalian COMT is widely expressed, and catalyzes the O-methylation of catecholamines, dopamine and estrogen. Human COMT is a 271 amino acid (aa) type II transmembrane (TM) protein. It contains a six aa cytoplasmic N-terminus, an 20 aa TM segment, and a 245 aa luminal/extracellular region that possesses a methyltransferase domain (aa 112-217). This TM form of COMT (termed MB-COMT) is generally associated with pre- and postsynaptic neurons plus glia, and is suggested to be very important in deactivating neurotransmitters, an activity that likely impacts cognition. There is also a 25-26 kDa COMT isoform (termed S-COMT) that utilizes an alternative start site at Met51. This isoform is cytoplasmic and generally found outside the CNS in cells such as mammary and intestinal epithelium, renal tubule epithelium, pancreatic beta -cells, monocytes and mast cells. S-COMT is believed to inactive toxic catechols (hydroxylated benzenes found in plant walls). MB-CORM exhibits high affinity:low capacity activity, while S-COMT shows low affinity:high capacity activity. Two additional alternative start sites have been reported. One starts at Met90, while a second possesses a start site 38 aa upstream of the MB-COMT start site. Over aa 52-271, human COMT shares 79% aa sequence identity with mouse COMT.

Long Name

Catechol O-Methyltransferase

Entrez Gene IDs

1312 (Human)

Alternate Names

catechol O-methyltransferase; catechol-O-methyltransferase; COMT; EC 2.1.1.6