Human Contactin-4 Isoform a Antibody

Catalog #: AF2205
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2205
AF2205-SP

All Contactin-4 Antibodies

Product Details

Citations (2)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Contactin-4 Isoform a Antibody Summary

Species Reactivity

Human

Specificity

Detects Contactin‑4 isoform a in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CNTN2, recombinant mouse CNTN3, and rhCNTN5 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Contactin-4 isoform a
Asp19-Gly1001
Accession # Q8IWV2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.25 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Contactin‑4 (Catalog # 2205-CN)

Neutralization

The enhancement of neurite outgrowth from E16-18 rat cortical neurons induced by rhCNTN-4 (400 ng/3 μL, drop/well) is significantly inhibited by the antibody at a concentration of 5 μg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Contactin-4

Contactin-4 (CNTN4), also known as BIG-2 (Brain-derived Immunoglobulin Superfamily molecule 2), is an axonal cell adhesion molecule that belongs to the contactin family, a subfamily of the Ig superfamily (1‑3). The contactin family comprises six members (CNTN1/F3, CNTN2/TAG-1, CNTN3/BIG-1, CNTN4/BIG-2, CNTN5/NB-2 and CNTN6/NB-3) and are characterized by the presence of six Ig-like domains, four fibronectin type III-like repeats, and a glycosylphosphatidylinositol (GPI)‑anchoring domain. Contactin family proteins exist as membrane-bound proteins, but can also be released as soluble proteins by GPI‑specific phospholipase D. Soluble Contactins are functionsl proteins that are able to promote neurite outgrowth. Human CNTN4 has been mapped to chromosome 3p26‑p25. Three alternative transcripts of CNTN4, encoding isoforms a, b, and c precursor proteins containing 1026, 282, and 698 amino acid (aa) residues, respectively, have been described. Human CNTN4 isoform a shares 94% aa sequence identity with its rat homolog. It also shares from 44‑66% aa sequence identity with other CNTN family members. CNTN family members display overlapping but distinct expression patterns. CNTN4 expression is detected in multiple organs including brains, pancreas, kidney, aorta, small intestine, thyroid, uterus and testis. However, expression of the 282 aa isoform b is primarily restricted to the brain. CNTN4 has been suggested to play important roles in the formation of neuronal networks during nervous sytems development. Disruption of CNTN4 has been implicated in the 3p deletion syndrome characterized by growth failure, developmental delay, and mental retardation. CNTN-4 expression is induced in human neuroblastoma tumor cells treated with retinoic acid and may be responsible for the the neuritogenic response of tumor cells to retinoids. R&D Systems' recombinant CNTN4 shows in vitro neurite outgrowth promoting activity when used as an immobilized substrate for hippocampal neuron outgrowth.

References

Fernandez, T. et al. (2004) Am. J. Genet. 74:1286.
Hasnford, L.M. et al. (2003) Cytogenet. Genome Res. 101:17.
Yoshihara, Y. et al. (1995) J. Neurobiology 28:51.

Entrez Gene IDs

152330 (Human); 269784 (Mouse)

Alternate Names

AXCAM; axonal-associated cell adhesion molecule; BIG-2; BIG-2Brain-derived immunoglobulin superfamily protein 2; CNTN4; contactin 4; Contactin4; Contactin-4; MGC33615; neural cell adhesion protein BIG-2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Contactin-4 Isoform a Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Contactin 4, -5 and -6 differentially regulate neuritogenesis while they display identical PTPRG binding sites
Authors: Oriane Mercati, Anne Danckaert, Gwénaëlle André-Leroux, Marco Bellinzoni, Laura Gouder, Kazutada Watanabe et al.
Biology Open
BIG-2 mediates olfactory axon convergence to target glomeruli.
Authors: Kaneko-Goto T, Yoshihara S, Miyazaki H, Yoshihara Y
Neuron, 2008-03-27;57(6):834-46.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC