Human Corin Antibody Summary
Ser67-Asn1042
Accession # Q9Y5Q5
Applications
This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human Corin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2209).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Corin DuoSet ELISA Kit (Catalog # DY2209) for convenient development of a sandwich ELISA or the Human Corin Quantikine ELISA Kit (Catalog # DCRN00) for a complete optimized ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Corin in HK2 Human Cell Line. Corin was detected in immersion fixed HK2 human kidney proximal tubule cell line using Rat Anti-Human Corin Monoclonal Antibody (Catalog # MAB2209) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Human Corin ELISA Standard Curve. Recombinant Human Corin protein was serially diluted 2-fold and captured by Rat Anti-Human Corin Monoclonal Antibody (Catalog # MAB2209) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Corin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2209) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Corin
Corin is a type II transmembrane serine protease expressed predominantly in cardiomyocytes. It converts pro arterial natriuretic peptide (pro-ANP) to ANP.
Product Datasheets
Citations for Human Corin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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MicroRNA-302 switch to identify and eliminate undifferentiated human pluripotent stem cells
Sci Rep, 2016-09-09;6(0):32532.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Functional Screening Identifies MicroRNA Regulators of Corin Activity and Atrial Natriuretic Peptide Biogenesis
Authors: Selvi Celik, Mardjaneh Karbalaei-Sadegh, Göran Rådegran, J. Gustav Smith, Olof Gidlöf
Molecular and Cellular Biology
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Used the antibody for floorplate progenitor cells. It worked really great.
