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Human Corin Quantikine ELISA Kit

Catalog #: DCRN00
5 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DCRN00
Control Products Available
QC67: Quantikine Immunoassay Control Set 1027 for Human Corin
Human Corin ELISA Standard Curve
2 Images
Product Details
Procedure
Citations (5)
FAQs
Supplemental Products
Reviews (1)
Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human Corin Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Homogenates (50 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity
23.7 pg/mL
Assay Range
75.0 - 4,800 pg/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human Corin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Corin Immunoassay is a 4.5 hour solid phase ELISA designed to measure human Corin in cell culture supernates, tissue homogenates, serum, and plasma. It contains NS0-expressed recombinant human Corin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Corin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Corin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 618 1327 2794 660 1378 2819
Standard Deviation 16.9 35.6 69.6 41.4 61.3 106.4
CV% 2.7 2.7 2.5 6.3 4.5 3.8

Recovery

The recovery of Corin spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=6) 96 88-101
EDTA Plasma (n=6) 94 87-99
Heparin Plasma (n=6) 97 88-107
Serum (n=6) 94 89-100

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Corin were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Corin ELISA Linearity

Scientific Data

N/A Human Corin ELISA Standard Curve View Larger

Human Corin ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Corin

Corin is a type II transmembrane serine protease expressed predominantly in cardiomyocytes. It converts pro-atrial natriuretic peptide (pro-ANP) to ANP.

Entrez Gene IDs:
10699 (Human)
Alternate Names:
ATC2; atrial natriuteric peptide-converting enzyme; Corin; corin, serine peptidase; EC 3.4.21; EC 3.4.21.-; heart specific serine proteinase; Heart-specific serine proteinase ATC2; MGC119742; pro-ANP-convertase; Pro-ANP-Converting Enzyme; PRSC; serine protease; Transmembrane protease serine 10
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human Corin Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Association of Preablation Plasma Corin Levels With Atrial Fibrillation Recurrence After Catheter Ablation: A Prospective Observational Study
    Authors: Zhao, Y;Yuan, X;Xie, Y;Yin, X;Liu, Y;Sun, Y;Gong, Y;Liu, J;Chen, F;
    Journal of the American Heart Association
    Species: Human
    Sample Types: Plasma
  2. Associations between potentially functional CORIN SNPs and serum corin levels in the Chinese Han population
    Authors: H Zhang, X Mo, Q Qian, Z Zhou, Z Zhu, X HuangFu, T Xu, A Wang, Z Guo, S Lei, Y Zhang
    BMC Genet., 2019-12-19;20(1):99.
    Species: Human
    Sample Types: Serum
  3. Sex-specific association between soluble corin and metabolic syndrome in Chinese adults
    Authors: H Li, Q Zhang, Y He, J Shi, W Hu, H Peng
    Hypertens. Res., 2019-02-14;0(0):.
    Species: Human
    Sample Types: Serum
  4. Serum Soluble Corin Deficiency Predicts Major Disability within 3 Months after Acute Stroke
    PLoS ONE, 2016-09-22;11(9):e0163731.
    Species: Human
    Sample Types: Serum
  5. Plasma soluble corin in patients with heart failure.
    Authors: Dong N, Chen S, Yang J
    Circ Heart Fail, 2010-01-08;3(2):207-11.
    Species: Human
    Sample Types: Plasma

FAQs

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Human Corin Quantikine ELISA Kit
By Anonymous on 11/22/2021
Sample Tested: human embryonic stem cell medium,human embryonic stem cell medium_floxp
Human Corin Quantikine ELISA Kit DCRN00