Human CRBN Antibody

Name: Human CRBN Antibody
Brand: R&D Systems
SKU: MAB9574
Rating: 5 (1 reviews)

Catalog #: MAB9574 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9574-100
MAB9574-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CRBN Antibody Summary

Species Reactivity

Human

Specificity

Detects human CRBN in immunocytochemistry and immunohistochemistry.

Source

Monoclonal Mouse IgG_2B Clone # 978015

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Synthetic peptide Human CRBN
Accession # Q96SW2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

1-25 µg/mL

See below

Immunocytochemistry

5-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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CRBN in RAW 264.7 Mouse Cell Line. CRBN was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Mouse Anti-Human CRBN Polyclonal Antibody (Catalog # MAB9574) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry

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CRBN in Human Testis. CRBN was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human CRBN Polyclonal Antibody (Catalog # MAB9574) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRBN

Cerbeblon (CRBN) is a substrate recognition component of an E3 protein ligase complex which mediates ubiquitination and proteasomal degradation of target proteins. In embryonic development, degradation of regulatory proteins is required for normal limb outgrowth. Thalidomide, a teratogenic drug prescribed to pregnant women in the 1950s, binds to CRBN in the E3 protein ligase complex. Despite its teratogenic effect, CRBN is used to treat multiple myeloma and complications of leprosy. Widely expressed, CRBN may play a role in memory and learning by regulating assembly and surface expression of large-conductance calcium-activated potassium channels in hippocampal neurons. Mutations in the CRBN gene is linked to the Mental Retardation, Autosomal Recessive2a (MR2A) form of mental retardation.

Long Name

Cereblon

Entrez Gene IDs

51185 (Human); 58799 (Mouse); 297498 (Rat)

Alternate Names

Cereblon; CRBN; DKFZp781K0715; MGC27358; MRT2; MRT2A; non-syndromic, autosomal recessive, 2A; protein cereblon; Protein X 0001