Human Creatine Kinase MM/CKMM Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Creatine Kinase MM/CKMM by Western Blot. Western blot shows lysate of human heart tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Creatine Kinase MM/ CKMM Monoclonal Antibody (Catalog # MAB5564) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Creatine Kinase MM/CKMM at approximately 40-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human Creatine Kinase MM/CKMM by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Creatine Kinase MM/ CKMM at approximately 51 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human Creatine Kinase MM/CKMM Monoclonal Antibody (Catalog # MAB5564). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Creatine Kinase MM/CKMM
Creatine kinase (CK) is a 40 kDa cytoplasmic enzyme that is integral to energy metabolism in striated muscle through the maintenance of ATP levels. It is a dimeric protein composed of tissue specific M (muscle) and B (brain) subunits. The homodimeric CKMM isozyme is predominantly expressed in differentiated skeletal and cardiac muscle fibers. The M subunit of human creatine kinase shares 97% aa sequence identity with the mouse and rat M subunit and 81% with the human B subunit.
Product Datasheets
Citation for Human Creatine Kinase MM/CKMM Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Characterization of LAMP1-labeled nondegradative lysosomal and endocytic compartments in neurons
Authors: XT Cheng, YX Xie, B Zhou, N Huang, T Farfel-Bec, ZH Sheng
J. Cell Biol., 2018-04-25;0(0):.
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