Human CRELD1 Antibody

Catalog #: MAB3128 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3128
MAB3128-SP

Cell Adhesion Mediated by CRELD1 and Neutralization by Human CRELD1 Antibody.

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Human CRELD1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CRELD-1 in ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 842314

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CRELD-1
Gln30-Glu362
Accession # Q96HD1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Neutralization

Measured by its ability to neutralize CRELD1-mediated adhesion of the ATDC5 mouse chondrogenic cell line. The Neutralization Dose (ND₅₀) is typically 0.15-0.75 μg/mL in the presence of 2 μg/mL Recombinant Human CRELD1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Adhesion Mediated by CRELD1 and Neutralization by Human CRELD1 Antibody. Recombinant Human CRELD1 (Catalog # 3128-CR), immobilized onto a microplate, supports the adhesion of the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human CRELD1 (2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CRELD1 Monoclonal Antibody (Catalog # MAB3128). The ND₅₀ is typically 0.15-0.75 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRELD1

CRELD1, also known as cirrin, is an integral membrane protein that functions as a cell adhesion molecule. It is synthesized as a 420 amino acid (aa) precursor with an N-terminal signal peptide, a large extracellular domain with two EGF-like repeats, 2 transmembrane domains separated by a very short cytoplasmic loop, and a short extracellular C-terminal domain. An alternatively spliced isoform with a unique C-terminus that lacks the transmembrane domains has also been cloned. Missense mutations in CRELD1 have been associated with atrioventricular septal defects. The N-terminal extracellular domain of human and mouse CRELD1 share 92% aa sequence identity.

Long Name

Cysteine-rich Protein With EGF-like Domains 1

Entrez Gene IDs

78987 (Human); 171508 (Mouse); 312638 (Rat)

Alternate Names

atrioventricular septal defect 2; AVSD2; CIRRIN; CRELD1; cysteine-rich with EGF-like domain protein 1; cysteine-rich with EGF-like domains 1; DKFZp566D213