Human CTCF Antibody

Catalog #: AF6369 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6369
AF6369-SP

Detection of Human CTCF by Western Blot.

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Human CTCF Antibody Summary

Species Reactivity

Human

Specificity

Detects human CTCF in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human CTCF
Met1-Ile154
Accession # P49711

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human CTCF by Western Blot. Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human CTCF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6369) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CTCF at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CTCF

CTCF (CCCTC-binding factor) is a 130‑140 kDa member of the CTCF Zn-finger protein family. Although its predicted MW is 83 kDa, it runs anomalously slow in SDS‑PAGE. CTCF is ubiquitously expressed, save for primary spermatocytes, and serves multiple functions. It regulates both gene activation and repression by binding to unmethylated DNA sequences. Structurally, it contains eleven Zn-finger motifs, some or all of which may be involved in DNA binding. This variation in number allows for different conformations and multiple interaction partners. Human CTCF is 727 amino acids (aa) in length. It contains two SUMOylation sites (Lys74 and Lys689) and eleven consecutive C2H2-type Zn-finger domains (aa 266‑577). There are multiple Ser/Thr phosphorylation sites. Two potential alternative start sites exist; one nine aa upstream of the standard start site, and another at Met570. A third splice variant shows a 62 aa substitution for aa 349‑727. Over aa 1‑154, human CTCF shares 99% aa identity with mouse CTCF.

Long Name

CCCTC-binding Factor

Entrez Gene IDs

10664 (Human); 13018 (Mouse); 83726 (Rat)

Alternate Names

11 zinc finger transcriptional repressor; 11-zinc finger protein; 11-Zinc Finger Protein2; CCCTC-binding factor (zinc finger protein); CCCTC-binding Factor; CTCF; CTCFL paralog; transcriptional repressor CTCF