Human CTRP4/C1qTNF4 Antibody

Catalog #: AF3456 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3456
AF3456-SP

Detection of CTRP4/C1qTNF4 in A172 Human Cell Line by Flow Cytometry.

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Human CTRP4/C1qTNF4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CTRP4/C1qTNF4 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) CTRP5 is observed and less than 1% cross-reactivity with rhCTRP1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CTRP4/C1qTNF4
Leu17-Leu329
Accession # Q9BXJ3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human CTRP4/C1qTNF4

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of CTRP4/C1qTNF4 antibody in A172 Human Cell Line antibody by Flow Cytometry.

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Detection of CTRP4/C1qTNF4 in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Goat Anti-Human CTRP4/C1qTNF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3456, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CTRP4/C1qTNF4

Cq1TNF4, also known as CTRP4, is a secreted protein and is one of at least 9 paralogs of Acrp30/Adiponectin. Proteins in this family contain a globular C-terminal domain similar to that of the C1q and TNF ligand family. C1qTNF4 is the only family member that lacks the collagen-like domain but has two C1q domains. Mature human C1qTNF4 shares 92.4% amino acid sequence homology with the corresponding region of mouse C1qTNF4.

Long Name

C1q And Tumor Necrosis Factor Related Protein 4

Entrez Gene IDs

114900 (Human); 67445 (Mouse); 311184 (Rat)

Alternate Names

C1q and tumor necrosis factor related protein 4; C1qTNF4; complement C1q tumor necrosis factor-related protein 4; complement-c1q tumor necrosis factor-related protein 4; CTRP4; CTRP4ZACRP4; ZACRP4