Human CX3CL1/Fractalkine Chemokine Domain PE-conjugated Antibody
Human CX3CL1/Fractalkine Chemokine Domain PE-conjugated Antibody Summary
Gln25-Arg339 (Ser199Asn) (predicted)
Accession # P78423
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CX3CL1/Fractalkine in NS0 Cell Line Transfected with His-tagged Human CX3CL1 by Flow Cytometry. NS0 cell line transfected with His-tagged human CX3CL1 was stained with Mouse Anti-His Tag APC-conjugated Monoclonal Antibody (Catalog # IC050A) and either (A) Mouse Anti-Human CX3CL1/Fractalkine Chemokine Domain PE-conjugated Monoclonal Antibody (Catalog # IC365P) or (B) Mouse IgG1Phycoerythrin Isotype Control (Catalog # IC002P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CX3CL1/Fractalkine
CX3CL1, also named neurotactin, is a novel chemokine identified through bioinformatics. CX3CL1 has a unique C-X3-C cysteine motif near the amino-terminus and is the first member of a fourth branch of the chemokine superfamily. Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. Human CX3CL1 cDNA encodes a 397 amino acid (aa) residue membrane protein with a 24 aa residue predicted signal peptide, a 76 aa residue chemokine domain, a 241 aa residue stalk region containing 17 degenerate mucin-like repeats, a 19 aa residue transmembrane segment and a 37 aa residue cytoplasmic domain. The extracellular domain of human CX3CL1 can be released, possibly by proteolysis at the dibasic cleavage site proximal to the membrane, to generate soluble CX3CL1. CX3CL1 mRNA has been detected in various tissues including the brain and heart. The expression of CX3CL1 was also reported to be up-regulated in endothelial cells and microglia by inflammatory signals. Membrane-bound CX3CL1 has been shown to promote adhesion of leukocytes. The soluble chemokine domain of human CX3CL1 was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse CX3CL1 was reported to chemoattract neutrophils and T-lymphocytes but not monocytes.
- Pan, Y. et al. (1997) Nature 387:611.
- Bazan, J.F. et al. (1997) Nature 385:640.
- Mackay, C.R. (1997) Current Biology 7:R384.
Product Datasheets
Citations for Human CX3CL1/Fractalkine Chemokine Domain PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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DNA Methylation Regulates the Differential Expression of CX3CR1 on Human IL-7Ralphalow and IL-7Ralphahigh Effector Memory CD8+ T Cells with Distinct Migratory Capacities to the Fractalkine.
Authors: Shin M, You S, Kang Y, Lee N, Yoo S, Park K, Kang K, Kim S, Mohanty S, Shaw A, Montgomery R, Hwang D, Kang I
J Immunol, 2015-08-14;195(6):2861-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Fractalkine-CX3CR1-dependent recruitment and retention of human CD1c+ myeloid dendritic cells by in vitro-activated proximal tubular epithelial cells.
Authors: Kassianos A, Wang X, Sampangi S, Afrin S, Wilkinson R, Healy H
Kidney Int, 2015-01-14;87(6):1153-63.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
CX3CL1/fractalkine is released from apoptotic lymphocytes to stimulate macrophage chemotaxis.
Authors: Truman LA, Ford CA, Pasikowska M, Pound JD, Wilkinson SJ, Dumitriu IE, Melville L, Melrose LA, Ogden CA, Nibbs R, Graham G, Combadiere C, Gregory CD
Blood, 2008-09-17;112(13):5026-36.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Functional adhesiveness of the CX3CL1 chemokine requires its aggregation. Role of the transmembrane domain.
Authors: Hermand P, Pincet F, Carvalho S, Ansanay H, Trinquet E, Daoudi M, Combadiere C, Deterre P
J. Biol. Chem., 2008-08-25;283(44):30225-34.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Regulated shedding of transmembrane chemokines by the disintegrin and metalloproteinase 10 facilitates detachment of adherent leukocytes.
Authors: Hundhausen C, Schulte A, Schulz B, Andrzejewski MG, Schwarz N, von Hundelshausen P, Winter U, Paliga K, Reiss K, Saftig P, Weber C, Ludwig A
J. Immunol., 2007-06-15;178(12):8064-72.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Fractalkine mediates natural killer-dependent antitumor responses in vivo.
Authors: Lavergne E, Combadiere B, Bonduelle O, Iga M, Gao JL, Maho M, Boissonnas A, Murphy PM, Debre P, Combadiere C
Cancer Res., 2003-11-01;63(21):7468-74.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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