Human CXCL1/2/3/GRO Pan Specific Antibody

Catalog #: MAB2761
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2761
MAB2761-SP

Product Details

Citations (1)

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CXCL1/2/3/GRO Pan Specific Antibody Summary

Species Reactivity

Human

Specificity

Detects human CXCL1/2/3 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) CXCL2 is observed and no cross-reactivity with recombinant human (rh) VIC, rmVIC, rhCCL3L1, rhCCL4L1, or recombinant canine IL-8 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 29702

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human CXCL3
Ala35-Asn107
Accession # P19876

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human CXCL1/GRO alpha /KC/CINC-1 (Catalog # 275-GR)
Recombinant Human CXCL2/GRO beta /MIP-2/CINC-3 (Catalog # 276-GB)
Recombinant Human CXCL3/GRO gamma /CINC-2/DCIP-1 (Catalog # 277-GG)
under non-reducing conditions only

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

Human peripheral blood mononuclear cells treated with PHA, fixed with paraformaldehyde, and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL1/2/3/GRO

CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha ) and GRO gamma (MIP-2 beta ), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators.

Alternate Names

CXCL1/2/3; FSP; GRO1; GROa; MGSA; MGSA-a; NAP-3; SCYB1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human CXCL1/2/3/GRO Pan Specific Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Mesothelial cells with mesenchymal features enhance peritoneal dissemination by forming a protumorigenic microenvironment
Authors: Yonemura, A;Semba, T;Zhang, J;Fan, Y;Yasuda-Yoshihara, N;Wang, H;Uchihara, T;Yasuda, T;Nishimura, A;Fu, L;Hu, X;Wei, F;Kitamura, F;Akiyama, T;Yamashita, K;Eto, K;Iwagami, S;Iwatsuki, M;Miyamoto, Y;Matsusaki, K;Yamasaki, J;Nagano, O;Saya, H;Song, S;Tan, P;Baba, H;Ajani, JA;Ishimoto, T;
Cell reports
Species: Mouse
Sample Types: Whole Cells
Applications: Mass Cytometry