Human CXCR6 Antibody

Catalog #: MAB699R Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB699R-100
MAB699R-SP

Detection of CXCR6 in HEK293 Human cell line transfected with Human CXCR6 and eGFP by Flow Cytometry.

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All CXCR6 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CXCR6 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CXCR6 in direct ELISAs.

Source

Recombinant Monoclonal Mouse IgG_2B Clone # 56811R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human CXCR6
Met1-Leu342
Accession # O00574

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CXCR6 in HEK293 Human cell line transfected with Human CXCR6 and eGFP by Flow Cytometry. HEK293 Human cell line transfected with either (A) Human CXCR6 or (B) irrelevant transfectant and eGFP, was stained with Mouse Anti-Human CXCR6 Monoclonal Antibody (Catalog # MAB699R) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of CXCR6 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) treated with 20 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) for 6 days or (B) untreated were stained with Mouse Anti-Human CXCR6 Monoclonal Antibody (Catalog # MAB699R) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD3 epsilon PE-conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCR6

CXCR6, also known as Bonzo and STRL33, is a G protein coupled chemokine receptor expressed on activated T cells and tumor-infiltrating lymphocytes. It binds soluble CXCL16 and membrane-anchored CXCL16 expressed on dendritic cells and macrophages.

Entrez Gene IDs

10663 (Human); 80901 (Mouse); 100124593 (Rat)

Alternate Names

Bonzo; BONZOG-protein coupled receptor bonzo; CD186 antigen; CD186; CDw186; chemokine (C-X-C motif) receptor 6; C-X-C chemokine receptor type 6; CXCR6; CXC-R6; CXCR-6; G protein-coupled receptor; STRL33; STRL33G-protein coupled receptor STRL33; TYMSTRCD186