Human Cyclin E2 Antibody

Catalog #: MAB7444 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7444
MAB7444-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Cyclin E2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Cyclin E2 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 718916

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Cyclin E2
Met1-Gln140
Accession # O96020

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Cyclin E2 in Human Cervical Cancer Tissue. Cyclin E2 was detected in immersion fixed paraffin-embedded sections of human cervical cancer tissue using Mouse Anti-Human Cyclin E2 Monoclonal Antibody (Catalog # MAB7444) at 1.2 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cyclin E2

Cyclin E2, also known as CCNE2, is an approximately 45-50 kDa protein that associates with the kinase Cdk2. This complex phosphorylates multiple substrates involved in regulation of cell cycle progression and the initiation of DNA replication. Cyclin E2 is required for G1/S phase progression and cell cycle reentry from G0 phase. Cyclin E2 most highly expressed in testis, thymus, and brain and is frequently upregulated in cancer. Cyclin E2 contains two cyclin box folds (amino acids 141-231 and amino acids 241-361). An alternate splice form lacks 45 amino acids (aa) within the first cyclin box fold. Within aa 1-140, human Cyclin E2 shares 90% aa sequence identity with mouse and rat Cyclin E2.

Entrez Gene IDs

9134 (Human); 12448 (Mouse); 362485 (Rat)

Alternate Names

CCNE2; CYCE2; Cyclin E2; G1/S-specific cyclin E2; G1/S-specific cyclin-E2