Human Cystatin D Biotinylated Antibody

Catalog #: BAF1202 Datasheet / COA / SDS

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BAF1202

All Cystatin D Antibodies

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Human Cystatin D Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Cystatin D in Western blots. In this format, approximately 10% cross-reactivity with recombinant human (rh) Cystatin C and rhCystatin SN is observed, less than 5% cross-reactivity with rhCystatin S is observed and less than 1% cross-reactivity with rhCystatin A, rhCystatin B and rhCystatin E/M is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Cystatin D (R&D Systems, Catalog # 1202-PI)
Thr29-Val142
Accession # CAA49838

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Cystatin D (Catalog # 1202-PI)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cystatin D

Cystatin D is a member of family 2 of the cystatin superfamily (1). In contrast to other members of family 2, Cystatin D has restricted tissue distribution and has been found only in saliva and tears. Two allelic variants (Arg46 and Cys46) are known in the human protein and they are not significantly different in their inhibitory activity against papain and cathepsins B, H, L and S (2). The functions of Cystatin D are largely unknown. However, Cystatin D has been shown to inhibit coronavirus replication at its physiological concentration (0.12‑1.9 μM) and has been suggested to play a protective role against proteases present in the oral cavity (3).

References