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Human Cystatin S Antibody

Catalog #: MAB1296
1 Citation Datasheet / COA / SDS
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MAB1296
MAB1296-SP
Product Details
Citations (1)
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Human Cystatin S Antibody Summary

Species Reactivity
Human
Specificity
Detects human Cystatin S in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) Cystatin SA is observed and no cross-reactivity with rhCystatin D, E, SN, rhFetuin A, rhFetuin B, or rhHPRG (Histidine-Proline-Rich Glycoprotein) is observed. In Western blots, approximately 15% cross‑reactivity with rhCystatins SA and SN (under reducing conditions only) is observed and no cross‑reactivity with rhCystatin A, B, C, D, E/M, rhFetuin A, rhFetuin B, or rhHPRG is observed.
Source
Monoclonal Mouse IgG2B Clone # 201805
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cystatin S
Ser21-Ala141
Accession # P01036
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Cystatin S (Catalog # 1296-PI)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Cystatin S (Catalog # 1296-PI), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cystatin S

Cystatin S is a member of family 2 of the Cystatin superfamily (1). Like Cystatins SA and SN, it is produced by the salivary gland and secreted largely in the submandibular/sublingual saliva (2). Unlike Cystatins SA and SN, Cystatin S is not a potent inhibitor of cysteine proteases such as papain and some cathepsins (3). Cystatin S is partially phosphorylated at Ser21 and Ser23 in saliva (4). The functions of Cystatin S are largely unknown. However, it is able to bind more calcium and bind more rapidly to carbonated apatite than cystatins SA or SN, indicating that Cystatin S may be involved in the mineral balance of the tooth (3).

References
  1. Abrahamson, M. (1994) Methods Enzymol. 244:685.
  2. Baron, A.C. et al. (1999) Oral. Dis. 5:344.
  3. Baron, A. et al. (1999) Oral Dis. 5:234.
  4. Isemura, S. et al. (1991) J. Biochem. 110:648.
Entrez Gene IDs
1472 (Human)
Alternate Names
CST4; cystatin 4; Cystatin S; Cystatin-4; cystatin-S; cystatin-SA-III; MGC71923; Salivary acidic protein 1

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Citation for Human Cystatin S Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Murine monoclonal antibody which can distinguish cystatins SA1 and SA2.
    Authors: Ito T, Komiya-Ito A, Okuda K, Minaguchi K, Saitoh E, Yamada S, Kato T
    Mol. Immunol., 2005-01-08;42(10):1259-63.
    Species: Human
    Sample Types: Recombinant Protein, Saliva
    Applications: ELISA Development, Western Blot

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