Human Cystatin S Antibody

Catalog #: AF1296
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1296
AF1296-SP

All Cystatin S Antibodies

Product Details

Citations (1)

Supplemental Products

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Cystatin S Antibody Summary

Species Reactivity

Human

Specificity

Detects human Cystatin S in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human (rh) Cystatin SA and rhCystatin SN is observed and 5% cross-reactivity with rhCystatin C and rhCystatin D is observed, and less than 1% cross-reactivity with rhCystatin A, rhCystatin B, rhCystatin E/M, rhFetuin A, and rhFetuin B is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Cystatin S
Ser21-Ala141
Accession # P01036

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Cystatin S (Catalog # 1296-PI)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Cystatin S (Catalog # 1296-PI), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cystatin S

Cystatin S is a member of family 2 of the cystatin superfamily (1).Like cystatins SA and SN, it is produced by the salivary gland and secreted largely in the submandibular/sublingual saliva (2). Unlike cystatins SA and SN, Cystatin S is not a potent inhibitor of cysteine proteases such as papain and some cathepsins (3). Cystatin S is partially phosphorylated at Ser21 and Ser23 in saliva (4). The functions of Cystatin S are largely unknown. However, it is able to bind more calcium and bind more rapidly to carbonated apatite than cystatins SA or SN, indicating that Cystatin S may be involved in the mineral balance of the tooth (3).

References

Abrahamson, M. (1994) Methods Enzymol. 244:685.
Baron, A.C. et al. (1999) Oral. Dis. 5:344.
Baron, A. et al. (1999) Oral Dis. 5:234.
Isemura, S. et al. (1991) J. Biochem. 110:648.

Entrez Gene IDs

1472 (Human)

Alternate Names

CST4; cystatin 4; Cystatin S; Cystatin-4; cystatin-S; cystatin-SA-III; MGC71923; Salivary acidic protein 1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Cysteine Proteases and Regulators

Citation for Human Cystatin S Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1