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Human Cytosol Nonspecific Dipeptidase (CNDP2)/CPGL Antibody

Catalog #: AF3560
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3560
AF3560-SP
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Cytosol Nonspecific Dipeptidase (CNDP2)/CPGL Antibody Summary

Species Reactivity
Human
Specificity
Detects human Cytosol Nonspecific Dipeptidase (CNDP2)/CPGL in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human CPGL-2 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Cytosol Nonspecific Dipeptidase (CNDP2)/CPGL
Ala2-Asp475
Accession # Q96KP4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Cytosol Nonspecific Dipeptidase (CNDP2)/CPGL (Catalog # 3560-ZN)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Cytosol Nonspecific Dipeptidase (CNDP2)/CPGL (Catalog # 3560-ZN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cytosol Nonspecific Dipeptidase (CNDP2)/CPGL

The human CNDP2 gene encodes cytosol non-specific dipeptidase, a member of the M20 family of metalloproteases (1, 2). Also known as glutamate carboxypeptidase-like protein 1 (CPGL), tissue carnosinase or peptidase A, CNDP2 is ubiquitously expressed throughout human tissues. Unlike CNDP1, CNDP2 has broad substrate specificity for dipeptides with free amino and carboxyl groups. The purified recombinant human CNDP2 migrates as three bands in SDS-PAGE under reducing conditions that correspond to the full length protein (54 kDa), the N-terminal fragment (33 kDa) and the C-terminal fragment (22 kDa). The initiator Met1 may be removed and Ala2 may be acetylated based on Accession # Q96KP4.

References
  1. Teufel, M. et al. (2003) J. Biol. Chem. 278:6521.
  2. Woessner, J. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp.1020, Academic Press, San Diego.
Entrez Gene IDs
55748 (Human)
Alternate Names
CN2; CN2cytosolic non-specific dipeptidase; CNDP dipeptidase 2 (metallopeptidase M20 family); CNDP dipeptidase 2; CNDP2; CPGL; CPGLcytosolic nonspecific dipeptidase; FLJ10830; HsT2298; PEPA; PEPAEC 3.4.13.18; Peptidase AGlutamate carboxypeptidase-like protein 1

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Citation for Human Cytosol Nonspecific Dipeptidase (CNDP2)/CPGL Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Protein folding stress potentiates NLRP1 and CARD8 inflammasome activation
    Authors: EL Orth-He, HC Huang, SD Rao, Q Wang, Q Chen, CM O'Mara, AJ Chui, M Saoi, AR Griswold, A Bhattachar, DP Ball, JR Cross, DA Bachovchin
    Cell Reports, 2023-01-16;0(0):111965.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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