Human DAN Antibody

Catalog #: AF955 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF955
AF955-SP

All DAN Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DAN Antibody Summary

Species Reactivity

Human

Specificity

Detects human DAN in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse (rm) DAN is observed and less than 1% cross-reactivity with recombinant chicken Caronte, rmGremlin, and recombinant human Cerberus 1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human DAN
Ala17-Asp180 (Ala36Arg)
Accession # Q5U0N4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human DAN Fc Chimera (Catalog # 955-DA)

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human brain (hippocampus) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Blockade of Receptor-ligand Interaction

In a functional ELISA, 6-24 µg/mL of this antibody will block 50% of the binding of 100 ng/mL of Recombinant Human BMP-4 (Catalog # 314-BP) to immobilized Recombinant Human DAN (Catalog # 955-DA) coated at 2 µg/mL (100 µL/well). At 100 μg/mL, this antibody will block >90% of the binding.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DAN

DAN (differential screening-selected gene aberrative in neuroblastoma) was initially identified as a gene whose expression is downregulated in src-transformed rat fibroblasts. Human DAN was isolated from a normal lung cDNA library using mouse DAN as a probe. DAN has now been shown to be a prototypical member of the DAN family of secreted glycoproteins that are putative antagonists for TGF-beta superfamily proteins. DAN family members share a cysteine-rich domain that is structurally related to the cysteine-knot motif found in TGF-beta superfamily ligands. There are at least five mammalian DAN family members including DAN, Gremlin/DRM, Cer1 (Cerberus-related), Dante and PRDC (protein related to DAN and cereberus). Additional DAN family members include Xenopus Cerberus, chick Caronte and C. elegans CeCan 1. The DAN family of proteins are thought to act as antagonists by binding TGF-beta family ligands and preventing their interactions with signaling receptor complexes. Recombinant human DAN preparations from R&D Systems have been shown to bind BMP-4 in a functional ELISA and to inhibit BMP-4 mediated bioactivity in ATDC 5 chrondrogenic cells. It is likely the various DAN family members and other TGF-beta BMP antagonists including Noggin, Chordin, Follistatin and TSG can selectively antagonize the activities of different subsets of TGF-beta superfamily ligands. These antagonists represent one of the many elaborate regulatory mechanisms that have evolved to control the bioactivities of the TGF-beta superfamily ligands.

References

Massage, J. and Y-G. Chen (2000) Genes & Development 14:627.
Perch, J.J.H. et al. (1999) Develop. Biol. 209:98.
Enomoto, H. et al. (1994) Oncogene 9:2785.

Long Name

Differential Screening-selected Gene Aberrant in Neuroblastoma

Entrez Gene IDs

4681 (Human); 17965 (Mouse)

Alternate Names

DAN domain family member 1; DAN; DAND1; MGC8972; NBL1; neuroblastoma suppressor of tumorigenicity 1; neuroblastoma, suppression of tumorigenicity 1; Protein N03; suppression of tumorigenicity 1; Zinc finger protein DAN