Human DCC Antibody

Catalog #: AF5884 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5884
AF5884-SP

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Human DCC Antibody Summary

Species Reactivity

Human

Specificity

Detects human DCC in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) ICAM‑1, rhICAM-2, rhICAM-3, rhMadCAM-1, rhPECAM-1, rhVCAM-1, and rhCEACAM-1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human DCC
Ser1323-Phe1447
Accession # P43146

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human DCC by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human DCC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5884) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for DCC at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DCC

Deleted in colorectal cancer (DCC) is a 170-190 kDa transmembrane glycoprotein that contains four Ig-like domains and six fibronectin type III repeats in its extracellular domain. DCC is expressed on axons during development where its binding to Netrin-1 regulates axon migration and myelination. It inhibits apoptosis when bound by Netrin-1 and can induce apoptosis in the absence of ligand. DCC also functions as an adhesion molecule and a tumor suppressor on intestinal epithelial cells. Deficiencies in DCC function are associated with the development and metastasis of many tumors. Within aa 1323-1447 of the cytoplasmic domain, human DCC shares 98% aa sequence identity with the mouse and rat DCC.

Long Name

Deleted in Colorectal Cancer

Entrez Gene IDs

1630 (Human); 13176 (Mouse)

Alternate Names

Colorectal cancer suppressor; CRC18; CRCR1; DCC; deleted in colorectal cancer protein; deleted in colorectal carcinoma; IGDCC1colorectal tumor suppressor; Immunoglobulin superfamily DCC subclass member 1; immunoglobulin superfamily, DCC subclass, member 1; netrin receptor DCC; Tumor suppressor protein DCC

Product Datasheets

Product Datasheet

COA

SDS

Product Specific Notices

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain alicense to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 5,939,271, 6,277,585, and other U.S. and international patents pending.