Human Dectin-2/CLEC6A Antibody

Catalog #: AF3114
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3114
AF3114-SP

Detection of Dectin‑2/CLEC6A in Human Monocytes by Flow Cytometry.

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Dectin-2/CLEC6A Antibody Summary

Species Reactivity

Human

Specificity

Detects human Dectin‑2/CLEC6A in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant mouse Dectin-2 alpha is observed and less than 1% cross-reactivity with recombinant human Dectin-1/CLEC7A is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Dectin‑2/CLEC6A
Thr46-Leu209
Accession # Q6EIG7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Dectin‑2/CLEC6A (Catalog # 3114-DC)

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Dectin‑2/CLEC6A in Human Monocytes by Flow Cytometry. Human blood-derived monocytes were stained with Goat Anti-Human Dectin-2/CLEC6A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3114, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dectin-2/CLEC6A

Dectin-2, also known as CLEC6A, CLECSF10, and NKCL, belongs to the C-type lectin family of transmembrane immune regulatory glycoproteins. Dectin-2, plus CLEC4A-E constitute a subgroup of molecules that exhibit approximately 40% amino acid (aa) sequence identity in their extracellular domains (ECD), and have a conserved cysteine spacing in their carbohydrate recognition domains (CRD) (1, 2). Mature human Dectin-2 is a type II transmembrane protein with a short cytoplasmic tail, a transmembrane segment, and a 168 aa ECD with a stalk region and one CRD (3, 4). Within the ECD, human Dectin-2 shares 71% and 75% aa sequence identity with bovine and mouse Dectin-2, respectively. An alternately spliced beta isoform has a deletion of portions of the transmembrane and cytoplasmic regions (5). Full length Dectin-2 is a 27 kDa molecule that is expressed on monocytes, tissue macrophages, and activated CD4⁺ T cells (4‑6). The CRD of Dectin-2 contains an EPN motif which is characteristic of calcium-dependent mannose-binding lectins. Dectin-2 selectively interacts with high mannose structures in the Man₉GlcNAc₂ configuration (7). It mediates the recognition of a variety of microorganisms, particularly the filamentous forms of yeast and fungii (7, 8). The short cytoplasmic tail does not contain signaling motifs but mediates association with the ITAM-containing Fc receptor gamma subunit on macrophages (8). Ligation of Dectin-2 induces tyrosine phosphorylation of the gamma subunit, activation of NF kappa B, and enhanced release of TNF-alpha and IL-1ra (8). Macrophage Dectin-2 is up‑regulated in vivo by inflammatory stimuli and UV-B irradiation (5, 6, 9). Dectin-2 is known to participate in UV-induced immunosuppression by interacting with CD4⁺CD25⁺ regulatory T cells, which then induce dendritic cells to release IL-4, IL-10, and TGF-beta (10).

References

Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
Kanazawa, N. et al. (2004) Immunobiology 209:179.
Flornes, L.M. et al. (2004) Immunogenetics 56:506.
Kanazawa, N. et al. (2004) J. Invest. Dermatol. 122:1522.
Gavino, A.C. et al. (2005) Exp. Dermatol. 14:281.
Taylor, P.R. et al. (2005) Eur. J. Immunol. 35:2163.
McGreal, E.P. et al. (2006) Glycobiology 16:422.
Sato, K. et al. (2006) J. Biol. Chem. 281:38854.
Bonkobara, M. et al. (2005) Photochem. Photobiol. 81:944.
Aragane, Y. et al. (2003) J. Immunol. 171:3801.

Entrez Gene IDs

93978 (Human); 56620 (Mouse)

Alternate Names

CLEC4N; CLEC6A; CLECSF10; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 10; C-type lectin domain family 6 member A; C-type lectin domain family 6, member A; C-type lectin superfamily member 10; DC-associated C-type lectin 2; dectin 2; Dectin2; Dectin-2; Dendritic cell-associated C-type lectin 2

Product Datasheets

Product Datasheet

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Related Research Areas

Citations for Human Dectin-2/CLEC6A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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A unique serum IgG glycosylation signature predicts development of Crohn's disease and is associated with pathogenic antibodies to mannose glycan
Authors: Gaifem, J;Rodrigues, CS;Petralia, F;Alves, I;Leite-Gomes, E;Cavadas, B;Dias, AM;Moreira-Barbosa, C;Revés, J;Laird, RM;Novokmet, M;tambuk, J;Habazin, S;Turhan, B;Gümü?, ZH;Ungaro, R;Torres, J;Lauc, G;Colombel, JF;Porter, CK;Pinho, SS;
Nature immunology
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
The Role of AIRE in the Immunity Against Candida Albicans in a Model of Human Macrophages
Authors: JAT de Albuque, PP Banerjee, A Castoldi, R Ma, NB Zurro, LH Ynoue, C Arslanian, MUW Barbosa-Ca, JEM Correia-De, FG Weiler, MR Dias-da-Si, M Lazaretti-, LA Pedroza, NOS Câmara, E Mace, JS Orange, A Condino-Ne
Front Immunol, 2018-03-21;9(0):567.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Confocal Microscopy, Western Blot