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Human DEP-1/CD148 Antibody

Catalog #: MAB1934
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1934
MAB1934-SP
Detection of Human DEP‑1/CD148 by Western Blot.
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Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DEP-1/CD148 Antibody Summary

Species Reactivity
Human
Specificity
Detects human DEP-1/CD148 in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 143-41
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Phytohemagglutinin-stimulated human peripheral blood mononuclear cells
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
Human peripheral blood lymphocytes
Immunoprecipitation
2.5 µg/500 µg cell lysate
HeLa human cervical epithelial carcinoma cell line, see our available Western blot detection antibodies
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human DEP‑1/CD148 antibody by Western Blot. View Larger

Detection of Human DEP‑1/CD148 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human DEP-1/CD148 Monoclonal Antibody (Catalog # MAB1934) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DEP-1/CD148 at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DEP-1/CD148

Density Enhanced Protein Tyrosine Phosphatase (DEP-1), also known as CD148, HPTP-eta, and PTP receptor type J (PTPRJ), is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. A large (220 kDa) glycoprotein found at the cell surface, DEP-1 levels are increased with high cell density (1). DEP-1 phosphatase activity is enhanced by basement membrane proteins (2), suggesting it is involved in regulating cell adhesion and contact interactions. High levels of expression dampen PDGF (3), VEGF (4), and T‑cell receptor (5) responses. DEP-1 is widely expressed in tissues, particularly ones forming epithelioid monolayers (6). In the immune system, DEP-1 is found on all cell lineages and is highest on granulocytes (7). Dep-1 is the mutated gene in the Susceptibility to Colon Cancer locus Scc1, which is altered in many human colorectal adenomas (8). Gene knockout mice lacking DEP-1 die at midgestation due to failures in cardiovascular development (9). DEP-1 dephosphorylates a variety of proteins, including the HGF (10), PDGF (11), and VEGF (4) receptors, and beta-catenin (12). The recombinant protein is the intracellular region of DEP-1 containing the catalytic domain.

References
  1. Ostman, A. et al. (1994) Proc. Natl. Acad. Sci. USA 91:9680.
  2. Sorby, M. et al. (2001) Oncogene 20:5219.
  3. Jandt, E. et al. (2003) Oncogene 22:4175.
  4. Lampugnani, M.G. et al. (2003) J. Cell Biol. 161:793.
  5. Baker, J.E. et al. (2001) Mol. Cell. Biol. 21:2393.
  6. Borges, L.G. et al. (1996) Circ. Res. 79:570.
  7. de la Fuente-Garcia, M.A. et al. (1998) Blood 91:2800.
  8. Ruivenkamp, C.A. et al. (2002) Nat. Genet. 31:295.
  9. Takahashi, T. et al. (2003) Mol. Cell. Biol. 23:1817.
  10. Palka, H.L. et al. (2003) J. Biol. Chem. 278:5728.
  11. Kovalenko, M. et al. (2000) J. Biol. Chem. 275:16219.
  12. Holsinger, L.J. et al. (2002) Oncogene 21:7067.
Long Name
Density Enhanced Receptor Protein Tyrosine Phosphatase
Entrez Gene IDs
5795 (Human); 19271 (Mouse); 29645 (Rat)
Alternate Names
CD148 antigen; CD148; Density-enhanced phosphatase 1; DEP1; DEP-1; DEP1EC 3.1.3.48; HPTP eta; HPTPeta; HPTP-eta; human density enhanced phosphatase-1; protein tyrosine phosphatase, receptor type, J polypeptide; protein tyrosine phosphatase, receptor type, J; Protein-tyrosine phosphatase eta; Protein-tyrosine phosphatase receptor type J; PTPRJ; receptor-type tyrosine-protein phosphatase eta; R-PTP-ETA; R-PTP-J; SCC1; susceptibility to colon cancer 1, mouse, homolog of

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Citations for Human DEP-1/CD148 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. A novel splice variant of the protein tyrosine phosphatase PTPRJ that encodes for a soluble protein involved in angiogenesis
    Authors: A Bilotta, V Dattilo, S D'Agostino, S Belviso, S Scalise, M Bilotta, E Gaudio, F Paduano, N Perrotti, T Florio, A Fusco, R Iuliano, F Trapasso
    Oncotarget, 2017-02-07;0(0):.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. The protein tyrosine phosphatase DEP-1/PTPRJ promotes breast cancer cell invasion and metastasis.
    Authors: Spring K, Fournier P, Lapointe L, Chabot C, Roussy J, Pommey S, Stagg J, Royal I
    Oncogene, 2015-03-16;34(44):5536-47.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Phosphorylation of DEP-1/PTPRJ on threonine 1318 regulates Src activation and endothelial cell permeability induced by vascular endothelial growth factor.
    Authors: Spring K, Lapointe L, Caron C, Langlois S, Royal I
    Cell Signal, 2014-02-28;26(6):1283-93.
    Species: Bovine, Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Systematic validation of specific phenotypic markers for in vitro polarized human macrophages.
    Authors: Ambarus C, Krausz S, van Eijk M, Hamann J, Radstake T, Reedquist K, Tak P, Baeten D
    J Immunol Methods, 2011-10-29;375(1):196-206.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Analysis of HLDA9 mAbs on plasmacytoid dendritic cells.
    Authors: Cabezon R, Sintes J, Llinas L, Benitez-Ribas D
    Immunol. Lett., 2010-10-07;134(2):167-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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