Human Desmoglein-4 Antibody

Catalog #: MAB6589 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6589
MAB6589-SP

Detection of Human Desmoglein-4 by Western Blot.

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All Desmoglein-4 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Desmoglein-4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Desmoglein-4 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Desmoglein-1, -2, or -3 is observed and approximately 40% cross-reactivity with recombinant mouse Desmoglein-4 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 683135

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Desmoglein-4
Glu24-Ala631
Accession # Q86SJ6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Desmoglein-4 by Western Blot. Western blot shows lysates of human skin tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Desmoglein-4 Monoclonal Antibody (Catalog # MAB6589) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Desmoglein-4 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Desmoglein-4

Desmoglein-4 (DSG4) is a 110-130 kDa type I transmembrane glycoprotein in the cadherin family of calcium-dependent cell adhesion molecules. Desmogleins interact with transmembrane Desmocollins to form the adhesive components of desmosomes on epithelial cells. Desmoglein-4 contains four cadherin repeats in its extracellular region and two desmoglein repeat domains in its cytoplasmic region. Alternate splicing generates an additional isoform with a substitution in the cytoplasmic region. Desmoglein-4 is expressed on keratinocytes and trichocytes in the epidermis and hair follicles. It is required for hair follicle development, and DSG4 mutations are associated with autosomal recessive hypotrichosis. Within amino acids 24-631 (which includes the 26 aa propeptide and the ECD), human Desmoglein-4 shares 82% aa sequence identity with mouse and rat Desmoglein-4.

Entrez Gene IDs

147409 (Human); 16769 (Mouse); 291754 (Rat)

Alternate Names

Cad13; Cadherin family member 13; CDGF13; CDHF13; CDHF13CDH family member 13; desmoglein 4; Desmoglein4; Desmoglein-4; DSG4; LAHdesmoglein-4