Human DGK-delta Antibody

Catalog #: MAB7426 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7426
MAB7426-SP

Detection of Human DGK‑δ by Western Blot.

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All DGK-delta Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DGK-delta Antibody Summary

Species Reactivity

Human

Specificity

Detects human DGF-δ in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 625423

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human DGF-δ
Ile570-Thr717
Accession # Q16760

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human DGK‑δ by Western Blot. Western blot shows lysates of TT human medullary thyroid cancer cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human DGK-d Monoclonal Antibody (Catalog # MAB7426) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DGK-d at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DGK-delta

Diacylglycerol kinase delta (DGK-δ) is a widely expressed 140 kDa member of the DGK family of kinases that phosphorylate the lipid diacylglycerol. DGK-δ contains a pleckstrin homology domain (aa 53-146), two zinc finger domains (aa 163-213 and aa 235-286), a catalytic domain (aa 317-451), and a sterile alpha motif (aa 1145‑1208). An isoform of DGK-δ with a shortened N-terminus is expressed predominantly in ovary and spleen. The two isoforms can form intracellular homooligomers or heterooligomers with each other. The oligomers are disrupted by PKC following phorbol ester stimulation. DGK-δ regulates PKC mediated signaling downstream of the EGF receptor. Within aa 570-717, human DGK-δ shares 82% aa sequence identity with mouse and rat DGK-δ.

Long Name

Diacylglycerol Kinase, delta 130 kDa

Entrez Gene IDs

8527 (Human)

Alternate Names

130 kDa diacylglycerol kinase; DAG kinase delta; DGKD; dgkd-2; DGKdelta; DGK-delta; diacylglycerol kinase delta; diacylglycerol kinase, delta (130kD); diacylglycerol kinase, delta 130kDa; Diglyceride kinase delta; FLJ26930; KIAA0145EC 2.7.1.107