Human DIDO1 Antibody

Catalog #: MAB6947 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6947
MAB6947-SP

Detection of Human DIDO1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DIDO1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human DIDO1 in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 734823

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human DIDO1
Met1-Ser117
Accession # Q9BTC0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human DIDO1 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear (Nuc) extracts. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human DIDO1 Monoclonal Antibody (Catalog # MAB6947) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DIDO1 at approximately 280 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DIDO1

DIDO1 (Death-inducer obliterator 1; also DIO-1, C20orf158 and DATF-1) is a 245 kDa (predicted) intracellular protein that belongs the DIO family of proteins. It is ubiquitous in expression, and apparently serves multiple functions, depending on its splice variant. The standard form is a nuclear protein that maintains the integrity of mitotic checkpoint protein BubR1. An absence of DIDO1 results in centrosome amplification and unequal chromatin segregation. The best known short form (an 83 kDa isoform in mouse), by contrast, induces apoptosis by promoting transcription of caspase 9. Human standard form DIDO1 is 2240 amino acid (aa) in length. It contains two NLSs (aa 165-193), one PHD-type Zn finger region (aa 270-320), a TFIIS domain (aa 670-790), an SPOC protein-interaction domain (aa 1057-1163), two Pro-rich segments (aa 1257-1286 and 1725-2034) and a C-terminal Arg-rich region (aa 2108-2214). There are least 23 utilized phosphoryation sites. Multiple splice forms exist. Variants are generated by short aa substitutions for aa 531-2240, 566-2240 and 1182-2240, possibly coupled to a 36 aa insertion after Pro387. Over aa 1-117, human DIDO1 shares 80% aa identity with mouse DIDO1.

Long Name

Death Inducer Obliterator 1

Entrez Gene IDs

11083 (Human); 23856 (Mouse); 362286 (Rat)

Alternate Names

BYE1; C20orf158; DATF1; DATF-1; DATF1DIDO3; death associated transcription factor 1; death inducer-obliterator 1; Death-associated transcription factor 1; death-inducer obliterator 1; DIDO1; DIO1; DIO1DIDO2; DIO-1hDido1; dJ885L7.8; DKFZp434P1115; FLJ11265; KIAA0333chromosome 20 open reading frame 158; MGC16140