Human Dihydrofolate Reductase/DHFR Antibody

Catalog #: AF7934 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7934
AF7934-SP

Detection of Human Dihydrofolate Reductase/DHFR by Western Blot.

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All Dihydrofolate Reductase/DHFR Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Dihydrofolate Reductase/DHFR Antibody Summary

Species Reactivity

Human

Specificity

Detects human Dihydrofolate Reductase/DHFR in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Dihydrofolate Reductase/DHFR
Met1-Asp187
Accession # P00374

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Dihydrofolate Reductase/DHFR by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line, HEK293T human embryonic kidney cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Dihydrofolate Reductase/DHFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7934) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Dihydrofolate Reductase/DHFR at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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Dihydrofolate Reductase/DHFR in MCF‑7 Human Cell Line. Dihydrofolate Reductase/ DHFR was detected in immersion fixed MCF-7 human breast cancer cell line using Sheep Anti-Human Dihydrofolate Reductase/ DHFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7934) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counter-stained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dihydrofolate Reductase/DHFR

DHFR (DiHydroFolate Reductase; also Tetrahydrofolate dehydrogenase) is a 21-23 kDa member of the dihydrofolate reductase family of enzymes. It is a ubiquitously expressed monomer, and considered to be a housekeeping gene. Housekeeping genes are those that play a role in multiple pathways, although not the same pathway(s) in all cells. DHFR participates in the reduction of dihydrofolate to tetrahydrofolate, a product that is subsequently used in the synthesis of purines and thymidylic acid that are used to generate both RNA and DNA. Within the cell, DHFR is known to exist in two pools: one contains DHFR bound to its own RNA where it acts as a transcriptional repressor, while another contains DHFR bound to NADPH. Human DHFR is 187 amino acids (aa) in length and possesses one DHFR domain (aa 4-185). Its mRNA binding motif is suggested to involve Cys6, Leu22, Glu30 and Ser118. There is one potential alternative start site found 75 aa upstream of the standard start site. Full length human DHFR (aa 1-187) shares 90% aa sequence identity with mouse DHFR.

Entrez Gene IDs

1719 (Human); 13361 (Mouse); 24312 (Rat)

Alternate Names

DHFR; DHFRP1; Dihydrofolate Reductase; DYR; EC 1.5.1.3