Human Dishevelled-3 Antibody

Catalog #: AF3754 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3754
AF3754-SP

Detection of Human and Mouse Dishevelled-3 by Western Blot.

1 Image

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Dishevelled-3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Dishevelled-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human Dishevelled-1 and recombinant human Dishevelled-2 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Dishevelled-3
His545-Gly654
Accession # Q92997

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human liver cancer tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human and Mouse Dishevelled-3 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Dishevelled-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3754) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Dishevelled-3 at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dishevelled-3

Dishevelled-3 is one of three mammalian multi-moduled Dishevelled proteins that have important roles in the canonical and non-canonical Wnt pathways. Human Dishevelled-3 is a 716 amino acid residue protein with an N-terminal DIX domain, a central PZD domain, and a DEP domain. Over the region used as immunogen, human Dishevelled-3 shares 99% amino acid sequence homology with mouse and canine Dishevelled-3.

Entrez Gene IDs

1857 (Human); 13544 (Mouse); 303811 (Rat)

Alternate Names

dishevelled 3 (homologous to Drosophila dsh); dishevelled, dsh homolog 3 (Drosophila); Dishevelled3; Dishevelled-3; DSH homolog 3; DVL3; KIAA0208dishevelled-3; segment polarity protein dishevelled homolog DVL-3