Human DISP2 Antibody

Catalog #: MAB8434 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8434
MAB8434-SP

Detection of Human DISP2 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DISP2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human DISP2 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 758204

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human DISP2
Arg192-Lys477
Accession # A7MBM2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human DISP2 by Western Blot. Western blot shows lysates of NCI-N87 human gastric carcinoma cell line, U-118-MG human glioblastoma/astrocytoma cell line, and COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human DISP2 Monoclonal Antibody (Catalog # MAB8434) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for DISP2 at approximately 152 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DISP2

DISP2 (Dispatched Homolog 2), also known as DISPB, belongs to the dispatched family of sterol-sensing proteins. DISP2 and the closely related DISP1 were initially identified in mice by their high homology with Dispatched, a protein required for long-range Hedgehog (Hh) signaling in Drosophila. Like DISP1, DISP2 is a multispan (12) transmembrane protein with both cytoplasmic N- and C-terminal tails. DISP2 contains a sterol-sensing domain (SSD) of 173 amino acids (aa), and may be involved in regulating the release of cholesterol-modified Sonic Hedgehog (Shh). Human DISP2 is 1401 aa in length, with three predicted N-linked glycosylation sites. Over aa 192-477, human DISP2 shares 72% and 73% aa identity with mouse and rat DISP2, respectively.

Long Name

Dispatched 2

Entrez Gene IDs

85455 (Human); 214240 (Mouse); 311324 (Rat)

Alternate Names

DISP2; Dispatched B; Dispatched Homolog 2 (Drosophila); DISPB; HsT16908; KIAA1742; Protein Dispatched Homolog 2